武威市某镇乙型肝炎感染者家庭内部隐匿性感染现状及其分子进化特征

The status quo and molecular evolution of occult infection in intrafamilial patients with hepatitis B virus infection in Wuwei

目的研究武威市某镇乙型肝炎病毒(HBV)家庭内部感染者中隐匿性HBV感染(occult hepatitis B virus infection,OBI)的流行现状及其分子进化特征。方法以2009年武威市某镇体检发现的HBs Ag阳性者为先证者,询问其家族史,签署知情同意书后,对此家庭内部人员进行流行病学调查,收集血样。采用ELISA检测HBs Ag、抗-HBs、HBe Ag、抗-HBe、抗-HBc;对所有血清样本用病毒DNA磁珠法试剂盒提取,而后巢式PCR扩增S区获得HBVDNA片段,测序后应用mega6.0进行进化树分析。结果共收集血清学标本80份;HBs Ag、抗-HBc、HBVDNA的阳性率分别为43.8%、78.8%、37.5%;OBI共有12例,感染率是26.7%(12/45)。结论在HBV感染家庭中,家庭内部OBI阳性率明显高于一般人群,基因型主要是C型、D型,其中基因型D型居多。

Objective To study the status quo and molecular evolution of occult infection in intrafamilial patients with hepatitis B virus infection in the city of Wuwei. Methods All the people who were found to be infected with HBs Ag during a physical examination in 2009 in a town in Wuwei were questioned for their HBV family history. After they signed informed consent,their blood was drawn and tested for hepatitis B surface antigen（ HBs Ag）,antibody to HBs Ag（ anti-HBs）,hepatitis B e-antigen（ HBe Ag）,antibody to HBe（ anti-HBe） and antibody to hepatitis B core antigen（ anti-HBc） using commercially available enzyme-linked immunosorbent assay（ ELISA）. DNA was extracted using magnetic Beads extraction kit from all the samples,and amplification was performed by nested PCR. Sequences obtained were analyzed by mega 6. 0. Results Of the 80 serum samples,the positive rate of HBs Ag,Anti-HBc,and HBV DNA was 43. 8%,78. 8% and 37. 5%,respectively. The rate of infection with intrafamilial occult hepatitis B was 26. 7%（ 12 /45）. Conclusions The prevalence of intrafamilial occult hepatitis B infection is significantly higher than in the general population. The main genotype is genotype C and genotype D in particular.