食管癌组织中PR-SET7的表达及其与临床特征、增殖细胞核抗原的相关性

Expression of PR-SET7 in esophageal carcinoma and its correlation with clinical features and PCNA index

目的探讨赖氨酸甲基转移酶(Lysine methyltransferase,KMT)PR-SET7在食管癌组织中的表达及其与临床特征、增殖细胞核抗原(proliferating cell nuclear antigen,PCNA)之间的相关性。方法选择2010-06至2011-02住院的食管癌患者32例,应用免疫组织化学法检测食管癌组织和癌旁组织中PR-SET7和PCNA的表达,并分析PR-SET7表达量与患者性别、年龄、细胞分化程度、TNM分期和淋巴结转移等临床特征的相关性,以及PR-SET7的表达情况和PCNA指数之间的相关性。结果 PR-SET7及PCNA均以核表达为主,癌组织中PR-SET7及PCNA的表达明显强于癌旁组织,其中PR-SET7高表达率为81.25%,癌旁组织中以低表达为主,高表达率为15.62%(P<0.01);PCNA指数癌组织中为68.12±16.03,癌旁组织38.22±20.56,两者比较差异有统计学意义(P<0.05)。PR-SET7在食管癌中的表达与肿瘤细胞分化程度、TNM分期和淋巴结转移有相关性(P<0.05),与患者的性别、年龄无相关性(P>0.05)。PR-SET7的表达程度和PCNA指数有相关性(rs=0.551,P=0.001)。结论食管癌组织中PR-SET7异常表达,其表达水平可能与肿瘤的进展、转移及预后密切相关;PR-SET7的表达与PCNA指数有显著相关性,对探讨其作用机制有一定意义。

Objective To investigate the expression of lysine methyltransferase（ KMT） PR-SET7 in esophageal carcinoma and its relationship with clinical features and proliferating cell nuclear antigen（ PCNA）. Methods Immunohistochemistry was used to detect the PR-SET7 and PCNA expressions in esophageal cancer tissues and adjacent normal tissues of 32 cases between June 2010 and February 2011. The correlations between the expression of PR-SET7 and patients＇ gender,age,degree of carcinoma tissue differentiation,TNM stages,lymph node metastasis and proliferation of tumor cells were analyzed. Results Immunohistochemistry results showed that PR-SET7 and PCNA were mainly expressed in nuclear. The expression of PR-SET7 and PCNA in cancerous tissues was significantly stronger than in adjacent tissues. The high expression rate of PR-SET7 was 81. 25%,but the expression was mostly low in adjacent tissues,where the high expression rate was 15. 62%（ P〈0. 01）. PCNA index in cancer tissues was 68. 12 ± 16. 03,normal tissues was 38. 22 ± 20. 56,both comparative difference was statistically significint（ P〈0. 05）. PR-SET7 protein levels in patients with esophagus carcinoma were correlated with invasive depth and lymph node metastasis（ P〈0. 05） rather than with age or gender（ P〉0. 05）. There was a significant correlation between the expression of PR-SET7 and PCNA index（ rs= 0. 551,P = 0. 001）.Conclusions There is an abnormal expression of PR-SET7 and PCNA during the development and progression of esophageal carcinoma. The expression level may be related to tumor progression,metastasis and prognosis. There is a significant correlation between PRSET7 and PCNA overexpression in cancer tissues,which is of significance for exploration of the mechanism.