急性髓系白血病患者诱导化疗前后血清巨噬细胞炎症蛋白-1α和干扰素γ诱导蛋白-10水平的变化及意义

Serum level and clinical significance of macrophage inflammatory protein-1α and interferon ;gamma inducible protein-10 in acute myelogenous leukemia

目的：研究急性髓系白血病（AML）患者诱导化疗前后血清巨噬细胞炎症蛋白-1α（MIP-1α）和干扰素γ诱导蛋白-10（IP-10）水平的变化，探讨其在AML中的临床意义。方法采用酶联免疫吸附法定量检测34例AML患者（观察组）、20例健康者（健康对照组）血清MIP-1α和IP-10的水平。结果观察组诱导化疗前血清MIP-1α和IP-10水平均明显高于健康对照组，差异有统计学意义（P＜0.05）。观察组诱导化疗后血清MIP-1α和IP-10水平显著降低，与本组诱导化疗前比较差异有统计学意义（P＜0.05），与健康对照组比较差异无统计学意义（P〉0.05）。AML患者诱导化疗1个疗程后有21例达完全缓解（CR），13例未达CR。CR组血清MIP-1α和IP-10水平与健康对照组比较差异无统计学意义（P＞0.05）；而未达CR组血清MIP-1α和IP-10水平显著高于健康对照组和 CR 组（P＜0.05）。CR 组与未达 CR 组 MIP-1α水平下降百分比分别为（32.51±10.34）%和（10.57±10.39）%，差异有统计学意义（P＜0.05）；CR组与未达CR组IP-10水平下降百分比分别为（45.94±13.68）%和（31.17±11.85）%，差异有统计学意义（P＜0.05）。线性相关分析表明，初发未治疗AML患者血清MIP-1α和IP-10水平呈显著正相关（r＝0.652，P＜0.05）。结论 AML患者不同状态下血清MIP-1α和IP-10水平不同，且两者具有显著相关性，联合检测有望在AML的病情监测、预后判断等方面提供依据。

Objective To study the serum level of macrophage inflammatory protein-1α（MIP-1α） and interferon gamma inducible protein-10 （IP-10） in acute myelogenous leukemia （AML） and clarify their clinical significance. Methods Enzyme-linked immunosorbent assay was used to detect the level of MIP-1α and IP-10 in serum samples from 34 AML patients（observation group） and 20 volunteers （normal control group）. Results The levels of MIP-1αand IP-10 in observation group before induction chemotherapy were significantly higher than those in normal control group （P〈0.05）. The levels of MIP-1αand IP-10 in observation group after induction chemotherapy were decreased, and significantly lower than those before induction chemotherapy （P〈0.05）. After treatment for one course, 21 patients reached complete remission （CR）, and 13 patients did not reach CR. The levels of MIP-1αand IP-10 in CR group had no significant difference compared with those in normal control group （P〈0.05）, but the levels of MIP-1αand IP-10 in none CR group were significantly higher than those in normal control group and CR group （P〈0.05）. The drop percentage of MIP- 1αlevels in CR group and none CR group was （32.51 ± 10.34）% and （10.57 ± 10.39）%, and there was significant difference （P〈0.05）. The drop percentage of IP-10 levelsin CR group and none CR group was（45.94 ± 13.68）% and （31.17 ± 11.85）%, and there was significant difference （P〈0.05）. Liner correlation analysis revealed that the levels of MIP-1αand IP-10 had significantly positive correlation in AML patients （r=0.652, P〈0.05）. Conclusions Different expressions of serum MIP-1α and IP-10 are found before and after induction chemotherapy AML patients, and there is significant correlation. Combined detection of serum MIP-1αand IP-10 may be used as an index to monitor clinical stages and prognosis.