不同种源玉竹多糖含量比较及相关性分析

Comparison and Correlation Analysis of Polysaccharose Contents in Polygonati Odorati Rhizoma from Different Provenances

目的:不同种源玉竹多糖含量比较及分析,为玉竹种质资源利用与良种选育提供依据。方法:收集全国主分布区玉竹野生种质资源21份,考察农艺性状、经纬度与海拔,用苯酚-硫酸法测定多糖含量,用Excel,SPSS,DPS软件进行数据分析。结果:供试玉竹种源间农艺性状与多糖含量存在极显著差异(P<0.01),湖南与四川种源多糖含量较高,其次为浙江种源;多糖含量与叶宽、根状茎直径、海拔相关性较低,在叶大而狭长,茎较粗,果实较大的低经纬度种源中较高;12个性状可简化为5个主成分,累计贡献率达85.44%;21个种源可划分为4个类群,类群Ⅰ植株较高大,茎较粗,果实大,叶片较多而狭长或宽厚,根状茎产量高,综合性状表现较好。结论:共筛选出8个优良种源,良种选育时,可优先在湖南与四川平昌种源中选择,其次为浙江临安、浙江磐安与吉林抚松种源。

Objective： Comparison and correlation analysis of polysaccharose contents in Polygonati Odorati Rhizoma from different provenances to provide the basis for superior provenance selection of Polygonati Odorati Rhizoma. Method： The 21 pieces of wild provenance resources of Polygonati Odorati Rhizoma were collected from the main distribution area of China. Their agronomic traits, longitude and latitude, height were measured and recorded. Polysaccharose content was detected by phenol-sulfuric acid method, and then the obtained data were analyzed by Excel, SPSS and DPS software. Result： There were extremely significant difference in agronomic traits and polysaccharose contents between Polygonati Odorati Rhizoma from different provenances （P 〈 0.01）. The polysaccharose contents in Hunan and Sichuan provenance were higher, followed by Zhejiang provenance. Polysaccharose contents had low correlations with width of leaves, diameter of rhizome and heights, however, the polysaccharose contents were higher in the lower longitude and latitude provenances from which the plants presented larger area and ratio of length and width of leaves, thicker stem and larger diameter of fruits. What＇s more, 12 characters tested were simplified to 5 principle components, with a cumulative contribution rate of 85.44%. 21 provenances could be divided into 4 groups and the comprehensive characters of the first group showed the best performance. Conclusion： The 8 superior provenances have been selected and more attention should be paid on Hunan and Sichuan Pingchang provenance, followed by Zhejiang Lin＇an, Zhejiang Pan＇an and Jilin Fusong provenances for superior variety breeding.