河南连翘不同极性部位的HPLC指纹图谱

HPLC Fingerprints of Different Polarity Fractions from Henan Forsythia Fructus

目的:建立河南连翘饮片不同极性部位的HPLC指纹图谱,比较不同提取部位化学成分之间的差异,为全面评价河南连翘饮片的质量提供参考。方法:将河南连翘饮片用75%乙醇制备总提取物后,分别用石油醚、乙酸乙酯、三氯甲烷、正丁醇、水依次萃取,浓缩至浸膏,减压干燥制得粉末样品,建立HPLC指纹图谱,并采用相似度评价系统软件进行数据分析。结果:连翘饮片石油醚部位HPLC指纹图谱确定了18个共有峰,指认了3个指纹峰;三氯甲烷部位HPLC指纹图谱共确定了25个共有峰,指认了4个指纹峰;乙酸乙酯部位HPLC指纹图谱共确定了20个共有峰,指认了3个指纹峰;正丁醇部位HPLC指纹图谱共确定了17个共有峰,指认了3个指纹峰;水部位HPLC指纹图谱共确定了17个共有峰,指认了2个指纹峰。连翘饮片同一极性部位指纹图谱相似度较高,不同极性部位之间化学成分有明显差异。结论:该实验方法准确、可靠、重复性高,所建指纹图谱可以全面反映连翘饮片的化学成分分布,为连翘饮片的整体质量评价提供参考。

Objective： To establish the HPLC fingerprints of different polarity fractions from Henan Forsythia Fructus, compare their chemical compositions between the different polarity fractions, and provide a reference for comprehensive evaluation of the quality of Henan Forsythia Fructus. Method： Henan Forsythia Fructus was extracted by 75% ethanol to obtain the total extract, and then it was extracted by petroleum ether, ethyl acetate, chloroform, n-butanol, and water respectively; concentrated to extractum, and dried under reduced pressure to obtain powder samples. The samples were analyzed by HPLC fingerprints, and the similarity evaluation software was used for data analysis. Result： Eighteen common peaks were determined in HPLC fingerprints of petroleum ether fraction, and 3 peaks of them were identified; 25 common peaks were determined in HPLC fingerprints of chloroform extract fraction, and 4 peaks of them were identified; 20 common peaks were determined in the HPLC fingerprints of ethyl acetate fraction, and 3 peaks of them were identified; 17 common peaks were determined in HPLC fingerprint of n-butyl alcohol fraction, and 3 peaks of them were identified ; 17 common peaks were determined in HPLC fingerprints of water extract, and 2 peaks of them were identified. The fingerprint similarity of the same polarity fractions of Forsythia Fructus was high, with significant differences in chemical compositions between different polarity fractions. Conclusion： The experimental method is accurate, reliable, and highly repeatable, and can fully reflect the distribution of the chemical composition of Forsythia Fructus, providing a reference for the comprehensive quality evaluation of Forsythia Fructus decoction pieces.