水母雪莲内生真菌Alterraria sp.的次级代谢产物鉴定

Secondary Metabolites of An Endophytic Fungus Alterraria sp.Isolated from Saussurea medusa

目的:研究藏族药水母雪莲内生真菌Alterraria sp.的次级代谢产物。方法:从植物水母雪莲中分离得到内生菌Alterraria sp.,在考察培养条件后,对该菌种进行了扩大培养。运用反复硅胶柱色谱,LH-20凝胶柱色谱、开放ODS柱色谱、高效液相色谱等方法,并结合液质联用分析技术,对菌液的总萃取物进行分离纯化;并根据化合物的理化常数测定及光谱学(1D和2D NMR,MS等)数据分析鉴定所得化合物结构。结果:对水母雪莲中分离得到的内生真菌交链孢菌属Alterraria sp.进行次级代谢产物的研究,共得到9个化合物,分别鉴定为spirotryprostatin A(1),对羟基苯甲醛(2),4-羟基苯基-2’-羟基丙酯(3),二氢对甲氧基肉桂酸(4),4-甲氧基苯甲酸(5),对羟基苯乙酸甲酯(6),4-羟基苯乙醇(7),对羟基苯甲酸(8),3-苯基丙酸(9)。细胞毒测试结果表明,从TPXa中分离得到的化合物1对肺癌细胞株A549具有较强的增殖抑制活性,其IC50为2.0 mg·L-1。结论:以上化合物均为首次从交链孢菌属真菌中分离得到。

Objective ： To study the secondary metabolites of an endophytic fungus Alterraria sp. isolated from Saussurea medusa. Method： Alterraria sp. was isolated from the medical plant S. medusa. The strain was cultured in a large-scale after the culture conditions had been optimized. The secondary metabolites were isolated by chromatography methods such as silica gel, Sephadex LH-20 columns, ODS flash column and HPLC, and the analysis of LC-MS. The structures of these compounds were identified by theirphysico-chemical constants and the NMR and MS data. Result： Nine compounds were isolated and identified as spirotryprostatin A （1）, 4- hydroxybenzoic aldehyde （ 2 ）, 4-hydroxyphenyl-2＇-hydroxypropanoate （3）, dihydro-p-methoxycinnamic acid （4）, 4-methoxybenzoic acid （ 5 ）, p-hydroxymethylphenylacetate （ 6 ）, 4-hydroxyphenyl ethanol （ 7 ）, 4- hydroxybenzoic acid （8）, 3-phenylpropanoic acid （9）. All compounds were tested for their inhibitory activities against human lung adenocarcinoma strain A-549. Compound 1 exhibited strong cytotoxic activity against A549 with ICs0 value of 2.0 mg ·L^-1. Conclusion： All the compounds were isolated from Alterraria for the first time.