水苏碱、胆碱及二者配伍对离体子宫收缩活动的调节作用

The regulatory effects of stachydrine,choline and their combination on thecontraction of isolated uterus

目的探讨水苏碱、胆碱及两者配伍对大鼠不同状态离体子宫平滑肌收缩活动的影响。方法自内蒙古医科大学动物实验中心取健康成年雌性未孕SD大鼠30只,建立正常大鼠离体子宫模型(n=15)和采用缩宫素制造痉挛离体子宫模型(n=15),采用累积加药的方式,依次分别给予从益母草注射液总生物碱分离提取的水苏碱溶液(40μl、40μl、80μl),胆碱溶液(40μl、40μl、80μl)及二者配伍溶液[40μl(水苏碱溶液20μl+胆碱溶液20μl)、40μl(水苏碱溶液20μl+胆碱溶液20μl)、80μl(水苏碱溶液40μl+胆碱溶液40μl)]。采用生理记录仪记录子宫收缩曲线,观察给药前后子宫收缩活动力、幅度和频率的变化。结果与给药前相比,水苏碱、胆碱单独刺激以及二者配伍刺激的各剂量均对大鼠正常离体子宫收缩活力、收缩张力、张力最小值和频率无明显影响,给药前后差异无统计学意义,但抑制率随着剂量的增大有降低的趋势。与造模前相比,造模后大鼠的离体子宫收缩活力、收缩张力和频率明显提高,造模前后差异有统计学意义;当水苏碱、胆碱单独刺激和二者配伍刺激后痉挛离体子宫平滑肌收缩活力、收缩张力和频率均显著低于造模后,而收缩张力最小值显著高于造模后,造模前后差异均有统计学意义。结论水苏碱、胆碱对子宫活动的影响与子宫所处的机能状态不同有关,二者配伍不存在明显的协同作用,但是二者均是益母草注射液中调节子宫活动的有效物质。

Objective To assess the effects of stachydrine,choline,and their combination from Leonurus japonicus Injection on uterus of rats.Methods Thirty SD rats were randomly divided into two groups：normal rat isolated uterus model （n =15） and spasmodic uterine model （n =15）.The model uteruses were successively given stachydrine （40 μl,40 μl,80 μl）,choline （40 μl,40 μl,80 μl）,or their combination [40 μl（stachydrine of 20 μl＋ choline of 20 μl）,40 μl （stachydrine of 20 μl ＋ choline of 20 μl）,80 μl （stachydrine of 40 μμl ＋ choline of 40 μl）] that isolated from Yimucao Injection total alkaloids by way of cumulative dosing.Routine physiological recording and recording for the uterine contraction curve were performed,and the change of uterine contraction vitality,amplitude,and frequency were recorded before and after administration.Results Compared with pre-administration,administration of stachydrine,choline alone,and their combination had no significant effect on contractility activity,contractility tension,minimum tension value,and contraction frequency in the normal uterus model.In addition,the inhibition rate decreased with the increase of dose.However,the uterine contraction activity,contraction tension and frequency were significantly increased in the isolated uterus model,and the spasm was observed in vitro after stimulation with stachydrine and choline alone.The contraction tension and frequency of uterine smooth muscle were significantly lower than those of the model group,and the minimum value of systolic tension was significantly higher than that of the model group.Conclusion Stachydrine and choline on uterine activity showed two-way adjustment effects depending on the state of the uterus.Stachydrine and choline had no significant synergistic or antagonistic effect,but both were effective substances in Leonurus japonicus Injection.