不同培养方法及培养密度对绵羊腔前卵泡发育的影响

Effect of Different Culture Methods and Densities on Sheep Preantral Follicles Development

通过对绵羊腔前卵泡培养方法的研究,能够探索出适宜绵羊腔前卵泡生长发育的培养条件,以及为进一步揭示绵羊卵泡生长发育的规律奠定一些基础。试验一通过使用微滴法和琼脂培养基法培养腔前卵泡,观察其对绵羊腔前卵泡生长的影响;试验二通过绵羊腔前卵泡单独培养与群体培养,探讨不同培养密度对绵羊腔前卵泡生长的影响。试验结果显示,使用微滴法与琼脂培养基法培养的绵羊腔前卵泡,在培养3天时,卵泡直径增加值和存活率差异均不显著(P>0.05);但培养6天时,琼脂培养基法培养的腔前卵泡的直径增加值和存活率均极显著高于微滴法培养的腔前卵泡(P<0.01);在使用琼脂培养基培养的条件下,对绵羊腔前卵泡进行单独培养和群体培养,在培养的6天时间内二者卵泡直径增加值和存活率差异均不显著(P>0.05)。试验结果表明,琼脂培养基法是较为适宜的绵羊腔前卵泡体外培养方法;培养密度对绵羊腔前卵泡体外培养并无显著影响。

The paper aims to investigate the optimal culture condition of sheep preantral follicles and provide evidence to explore the law of sheep follicle growth by studying culture methods. Experiment 1： traditional culture method and agar medium culture method were used to investigate the influence on the growth of sheep preantral follicles; experiment 2： sheep preantral follicles cultured alone and cultured in group were used to investigate the influence of different culture densities on the growth of sheep preantral follicles. The results showed that there was no significant difference between traditional method and agar medium method on the increase of diameter and survival rate after 3 days culture （/9〉0.05）; but after 6 days culture, the increase of diameter and survival rate of preantral follicles cultured by agar medium was extremely and significantly higher than the follicles cultured by traditional method （P〈0.01）; when cultured by agar medium, during the 6 days, there was no significant difference between follicles cultured alone and follicles cultured in group on the increase of diameter and survival rate （P〉0.05）. The results confirmed that agar medium culture method was qualified to sheep preantral follicles cultured in vitro; there was no significant influence of density on sheep preantral follicles cultured in vitro.