宫颈癌标本中HPV16的E6、E7和LCR的变异研究

Variation study of E6,E7 and LCR of HPV16 in cervical cancer

目的:探究分析E6、E7和LCR(long control region,LCR)在宫颈癌标本中HPV16中的变异情况。方法:随机选取我院病理实验室于2015年1月至2016年1月期间留存的HPV16阳性宫颈癌标本100例为研究对象,分别采用PCR技术进行E6、E7和LCR片段的扩增处理,并采用DNA序列进行PCR扩增产物的序列测定,分析E6、E7和LCR的变异表现。结果:E6基因中最常见变异为T350G(67.27%),E7基因中最常见变异为T789C(72.67%),LCR最常见变异为G7521A(90.90%),LCR区中出现G7799A、A7636C、C13T、C7678T新变异,E7区高度保守,YY1转录因子结合点是LCR变异的主要集中点。结论:宫颈癌标本中HPV16存在E6、E7和LCR变异情况,分析高危型HPV变异有助于宫颈癌HPV的早期诊断,可将其应用于宫颈癌防治的疫苗设计中,具有广泛的临床应用前景。

Objectives： To explore variation of E6,E7 and LCR（ long control region,the LCR） of HPV16 in cervical cancer. Methods： 100 HPV16 positive cervical cancer specimens in our hospital from January 2015 to January 2015 were randomly selected. By PCR technology,E6,E7 and LCR fragment respectively received amplification processing. DNA sequence was used for PCR amplification product sequence determination to analyze the variation of E6,E7 and LCR. Results： The most common variant for E6 gene was T350G（ 67. 27%）,the most com-mon variant for E7 gene was T789C（ 72. 67%） and the most common variant for the LCR was G7521A（ 90. 90%）. There were G7799 A,A7636C,C13 T,C7678T new variation in LCR area. E7 area was highly conservative. YY1 transcription factors combining site was the main focus of LCR variation. Conclusion： There are E6,E7 and LCR variation in HPV16 of cervical cancer specimens. Analysis of high-risk type of HPV variants can contribute to the early diagnosis of cervical cancer HPV,which can be used in the cervical cancer vaccine design,with wide prospect of clinical application.