6p21.1和7p15.3基因多态性与宫颈癌发生风险的研究

Two polymorphisms at 6p21.1 and 7p15.3 and the risk of cervical cancer

目的:探讨6p21.1 rs2494938和7p15.3 rs2285947基因多态性与宫颈癌发生风险的关联性。方法:以571例宫颈癌患者和657例非宫颈癌患者(对照组)为研究对象,用Taq Man MGB(minor grove binder)探针对6p21.1 rs2494938多态位点和7p15.3 rs2285947多态位点进行基因分型,分析不同基因型与宫颈癌发生风险的关联性。采用非条件Logistic回归分析统计该多态位点与宫颈癌遗传易感的关联性,计算相对危险度的比值比(OR)及95%置信区间(CI)。结果:rs2494938多态位点突变型GA和AA基因型频率在病例组和对照组的分布无显著差异(P=0.848)。rs2285947多态位点突变型GA和AA基因型频率在病例组和对照组的分布有显著差异(P=0.028);合并突变基因型(GA+AA)与野生型GG相比宫颈癌发生风险显著下降(OR=0.77,95%CI:0.62~0.97,P=0.025)。结论:rs2494938多态性与宫颈癌发生风险无显著关联,rs2285947多态性与宫颈癌发生风险有显著关联。

Objective：To investigate the correlation between 6p21.1 rs2494938 and 7p15.3 rs2285947 and risk of cervical cancer.Methods：A total of 571 cervical cancer patients and 657 cancer-free controls were recruited in the study. The genotypes of 6p21.1rs2494938 and 7p15.3 rs2285947 were detected by Taq Man MGB probe method. The correlation between the SNPs and the susceptibility to cervical cancer was evaluated using unconditional logistic regression analysis,and the relative risk of odds ratio（OR）and 95% confidence interval（CI） were calculated. Results：There was no significant association between the frequencies of GA or AA among the case and control groups at SNP rs2494938（P=0.848）. There was significant association between the frequencies of GA or AA among the case and control groups at SNP rs2285947,（P =0.028）,and significant reduction was found in the risk of cervical cancer between all mutation genotype（GA ＋ AA）and wild type genotype GG（OR=0.77,95%CI：0.62-0.97,P=0.025）. Conclusion：There was no significant association between the rs2494938 pleiotropy and cervical cancer susceptibility,and there was significant association between the rs2285947 pleiotropy and cervical cancer susceptibility.