叶片气孔保卫细胞周长用于鉴定黄花蒿倍性的研究

Leaf Stomatal Guard Cell Perimeter Used for Identification of Ploidy of Artemisia annua L.

以二倍体、四倍体及经秋水仙素处理的黄花蒿植株为试材,采用整体透明技术制备黄花蒿叶片样品,对不同倍性植物的叶片气孔保卫细胞长轴长、短轴长及其周长进行考察,探讨气孔保卫细胞大小与植株倍性的相关性。结果表明:以10%氢氧化钾溶液为透明剂,透化时间为1.5h的透化效果最好;二倍体与四倍体植物的气孔保卫细胞长轴长、短轴长及其周长差异显著,其周长范围分别为57.19~70.33μm和83.36~91.99μm;利用二倍体与四倍体的周长分布在不同区间范围,可以对秋水仙素处理的黄花蒿植株进行快速的初步倍性鉴定。

The diploid,tetraploid and colchicine treated Artemisia annua L.plants were used as the experimental materials.The integral transparent technique was applied to prepare the sample of the leave of Artemisia annua L.The long axis and short axis of stomatal guard cells of different ploidy plants were studied as well as the relationship between stomatal guard cell size and plant ploidy.The results showed that the optimal permeability was obtained by using 10% potassium hydroxide solution as the transparent agent and with the permeabilization time of 1.5hours.There were significant difference between the length of long axis,short axis length and perimeter of stomatal guard cells of diploid and tetraploid plants,which perimeter range from 57.19-70.33μm and 83.36-91.99μm.The rapid preliminary ploidy identification of colchicine treated Artemisiaa nnua L.plants was studies via the perimeter distribution in different interval range.