响应面法优化白薇抑菌物质超声提取工艺

Optimization of ultrasound extraction of antibacterial material from Cynanchum atratum Bunge with response surface methodology

为研究白薇抑菌物质的提取工艺条件,实验以白薇为原料,意大利青霉菌为供试菌,抑菌圈直径为指标,采用超声波辅助浸提白薇抑菌物质。在单因素实验基础上,研究响应面分析法(Response Surface Methodology,RSM)优化超声波辅助提取白薇抑菌物质的工艺参数。结果表明:白薇抑菌物质的最佳提取工艺为乙醇浓度50%,浸提时间2 h,液料比31,浸提温度69℃,在此条件下,白薇提取液对意大利青霉抑菌圈直径达到40.58 mm,与理论最优值40.81 mm接近。

In order to study the conditions of extracting antibacterial material from Cynanchum atratum,C.atratum was used as material ,the diameter of inhibition zone and Penicillium italicum worked as an indicator and bacterial strain in this experiment respectively,beside these ultrasound-assisted extraction was used to antibacterial material from C. atratum. On the basis of single factor tests, the method of response surface methodology was used to optimize ultrasound - assisted extraction of antibacterial material form C. atratum. The result indicated that the optimal conditions to extract antibacterial material from C.atratum were that ethanol concentration was 50%, extraction time was 2 h,liquid-to-material ratio was 31 and extraction temperature was 69 ℃. Under these conditions, the inhibitory effect against P. italicum by C.atratum extracts was 40.58 mm, close to the theoretically predicted value of 40.81 mm.