离子液体提取医药级磷脂酰胆碱的研究

Study on extraction of pharmaceutical grade phosphatidylcholine by ionic liquid

以大豆粉末磷脂为原料,采用混合溶剂萃取技术,研究了医药级磷脂酰胆碱的制备方法。首先通过单因素实验考察了混合溶剂1-乙基-3-甲基咪唑六氟磷酸盐([Emim]PF6)-甲醇的溶剂比、料液比、萃取温度、萃取时间等因素对提取效果的影响,并在此基础上进行优化。响应面结果表明:萃取时间60.12 min,[Emim]PF6-甲醇混合溶剂比2.95∶1(v/v),料液比1∶7.50(g/m L),萃取温度30.53℃,磷脂酰胆碱的纯度为87.65%,提取率为70.76%。再将磷脂酰胆碱经过氧化铝色谱柱纯化,最终产品的纯度可达98.85%。

The phosphatidylcholine was purified from soybean lecithin powder by mixed solvent.The influence factors（ mixing solvent ratio of 1 - ethyl-3 - methylimidazolium hexafluorophosphate （ [ Emim ] PF6 ） and methanol, solidliquid ratio, extracting temperature and extracting time）of separation were investigated by single factor experiment.The purified phosphatidylcholine products（with purity 87.65% and extracting yield 70.76% ）were obtained based on response surface experiment at the conditions as follows：extracting time 60.12 rain, mixing solvent ratio of[ Emim ] PF6 and methanol 2.95：1 （ v/v）, solid-liquid ratio 1 ： 7.50 （ g/mL ） , and extracting temperature 30.53 ℃ . The phosphatidylcholine was purified by aluminum column chromatography and the purity of final product was 98.85%.