玄参内生真菌分离、鉴定及系统发育分析

Identification and phylogenetic analysis of endophytic fungi isolated from Scrophularia ningpoensis

分别从武隆产区玄参根、主茎、侧枝、叶片和重庆市区栽培玄参侧枝中分离内生真菌,根据形态学特征和26Sr DNA D1/D2测序结果对其进行鉴定,并进行了种群多样性分析,对优势属炭疽菌属内生真菌构建了系统发育树。试验结果显示:真菌种群多样性指数,武隆玄参叶片>主茎=侧枝>根,重庆玄参侧枝>武隆玄参侧枝。共计分离得到内生真菌58株,绝大部分是各种植物病原菌,其优势属为刺盘孢属Colletotrichum,其中绝大多数是C.gloeosporioides和C.boninense。叶片和子芽可能是内生菌侵染的主要途径;内生菌和病原菌可能相互转化,受环境、基因型等多种因素影响。

The endophytic fungi from root, main stem, branch and leaf of Scrophularia ningpoensis were isolated and identified from Wulong and Chongqing, and the population diversity analysis and phylogenetic analysis were followed. The result indicated that, as to population diversity index, S. ningpoensis from Wulong： leaf 〉 main stem = branch 〉 root, branch from Chongqing 〉 branch from Wulong. Fifty-eight endophytic fungi were obtained, most of which were the pathogens of the plant. Colletotrichum was the prevailing ge-nus, of which C. gloeosporioides and C. boninense were the prevailing strains. Leaf and seedlings might be the main path of infection. Endophytic fungi and pathogen might convert to each other, influenced by such factors as environment, genotype et al.