菊苣提取物对高尿酸血症大鼠肾脏果糖转运体9表达的影响

Effect of Chinese herb chicory extract on expression of renal transporter Glut9 in rats with hyperuricemia

该研究给予雄性SD大鼠10%果糖饮水建立高尿酸血症模型,将其分为正常组、模型组、苯溴马隆组(20 mg·kg^(-1)·d^(-1))、菊苣提取物高、中、低剂量组(5,7.5,10 g·kg^(-1)·d^(-1)),连续42 d。分别测定血尿酸、血肌酐、尿素氮、尿尿酸,计算肾脏尿酸清除率;免疫组化法、逆转录实时荧光定量PCR(RT-q PCR)法分别检测果糖转运体9(glucose transporter family member 9,Glut9)在大鼠肾脏的蛋白及基因的表达水平。探讨中药菊苣提取物降尿酸作用及对肾脏Glut9表达的影响。结果显示菊苣提取物能降低高尿酸血症大鼠血尿酸水平,增加肾脏尿酸清除率,降低肾脏Glut9的蛋白表达,抑制肾脏尿酸重吸收,从而促进肾脏尿酸排泄。

Sixty SD male rats were randomly divided into normal group, model grotlp, benzbromarone group（20 mg · kg^-1· d^-1 ） , chicory extract high dose, middle dose and low dose groups （5, 7.5, 10 g·kg· d^-1 ）. The rats in normal group were given with water, and the rats in other groups were given with 10% fructose solution to establish hyperuficemia models. All the rats were sacri- ficed on the 42th day. Then their serum uric acid（SUA） , serum creatinine（CRE） , urea nitrogen（BUN） and urinary uric acid（UUA） levels were detected to calculate the clearance rate of uric acid in kidney（CUA）. Meanwhile, the protein and gene expression levels of renal glucose transporter family member 9 （ Glut9 ） were detected by immunohistoehemieal and Real-time quantitative reverse transcrip- tion-polymerase chain reaction（RT-qPCR） methods. The effects of Chinese herb chicory extract on expression of renal Glut9 and de- creasing uric acid were explored in this study, and the results showed that chicory extract could reduce SUA level in rats with hyperuri- cemia, increase renal CUA, decrease the protein expression of renal Glut9, inhibit uric acid re-absorption in kidney, and thus promote renal uric aeid exeretion.