组织芯片检测溶血磷脂酸受体2蛋白在胃癌组织中的表达及其临床意义

LPA2 Expression in Gastric Cancer and Its Clinical Significance by Tissue Chip Detection

目的探讨溶血磷脂酸受体2(LPA2)蛋白在胃癌组织中的表达水平及其与临床病理特征的关系。方法选取52例手术切除胃癌组织标本、22例上皮内瘤变标本及13例正常胃黏膜标本为研究对象,采用免疫组织化学SP法检测上述3组标本组织芯片中LPA2的表达水平;分析LPA2表达水平与胃癌患者临床病理特征(年龄、性别、临床分期、部位、浸润深度、肿瘤分级和淋巴结转移情况)是否存在相关性。结果 LPA2主要表达于细胞膜和胞浆,呈棕褐色颗粒状,在胃癌组织、上皮内瘤变及正常胃粘膜中的阳性表达率分别为73.1%(38/52)、54.5%(12/22)和46.2%(6/13),癌组织中的阳性率显著高于上皮内瘤变及正常胃粘膜(P<0.05);LPA2阳性表达与患者年龄、性别、肿瘤部位、分化程度无关(P>0.05),而与肿瘤浸润深度、淋巴结转移情况及临床分期有关(P<0.05)。浸润浆膜层、存在淋巴结转移和晚期患者LPA2阳性率显著高于未浸润浆膜层、无淋巴结转移和早期患者。结论LPA2在胃癌组织中阳性表达率较高,并与肿瘤的浸润深度、淋巴结转移及临床分期有关,可作为胃癌预后的潜在生物学标志物。

Objective To evaluate the LPA2 expression in gastric cancer and its clinical significance by tissue chip detection. Methods 52 gastric cancer tissues,22 intraepithelial neoplasia specimen and 13 normal gastric tissues were collected and were tested the LPA2 expression by tissue microarray immuno histochemistry assay. The LPA2 expression pattern and patients＇ clinical pathological features such as age,gender,location invasion depth and lymph node metastasis were analyzed. Results The LPA2 was mainly expressed in the cell membrane and patina with brown granular. The positive expression rates were73. 1%（ 38 /52）,54. 5%（ 12 /22） and 46. 2%（ 6 /13） for cancer tissue,intraepithelial neoplasia and normal gastric mucosa respectively with statistical difference（ P〈0. 05）. The positive rate of cancer tissue was significantly higher than that of intraepithelial neoplasia and normal gastric mucosa（ P〈0. 05）.The LPA2 positive expression was not associated with age,gender,tumor location and tumor grading（ P〉0. 05）,but was related with tumor invasion depth,lymph node metastasis and clinical stage（ P〈0. 05）.The LPA2 positive rates of patients with infiltrating serosa and lymph node metastasis in the advanced stage were significantly higher than those of patients without infiltrating serosa and lymph node metastasis in the early stage. Conclusion The LAP was highly expressed in gastric cancer correlating with tumor invasion depth,lymph node metastasis and clinical stages. It could be a potential biomarker for prognosis of gastric cancer.