摘要
目的:建立不同批次白芍甘草药对醇提物的指纹图谱。方法:采用HPLC法,Diamonsil(钻石)C18ODS(200mm×4.6 mm,5μm)色谱柱,流动相:乙腈-0.1%磷酸水梯度洗脱,波长切换,流速为1.0 m L/min。结果:建立了白芍甘草药对醇提物的指纹图谱,其指标性成分甘草酸含量显著升高,芍药内酯苷、芍药苷略有降低。结论:白芍甘草药对配伍后有效成分含量增加,其醇提物的指纹图谱相似度较好。
Objective: The establish fingerprints of different batches of Paeoniae Radix alba and Glycyrrhizae Radix et Rhizoma herb pairs alcohol extract. Methods: Use Diamonsil( diamond) C18ODS( 200 mm × 4. 6 m,5 μm) chromatography column. Mobile phase: acetonitrile-0. 1% phosphoric acid water,gradient elution,wavelength switching,flow rate 1. 0 m L / min. Results: The fingerprints of Paeoniae Radix alba and Glycyrrhizae Radix et Rhizoma alcohol extract was established. The index component of glycyrrhizic acid increased significantly. Albiflorin and paeoniflorin decreased slightly. The fingerprints of Paeoniae Radix alba and Glycyrrhizae Radix et Rhizoma were as basic two drugs and no new peaks obviously. Conclusion: Active ingredient contents after compatibility of Paeoniae Radix alba and Glycyrrhizae Radix et Rhizoma herb pairs increase. The alcohol extract fingerprints have high similarity.
出处
《辽宁中医杂志》
CAS
北大核心
2017年第3期563-565,I0001,共4页
Liaoning Journal of Traditional Chinese Medicine
基金
辽宁省科学技术基金(2013020176)
关键词
白芍
甘草
药对
指纹图谱
Paeoniae Radix alba
Glycyrrhizae Radix et Rhizoma
herb pairs
fingerprints