佩兰对2型糖尿病合并脂代谢紊乱大鼠肝脏DGAT2表达的影响

Efficacy of Eupatorium fortunei Turcz. Extract Ameliorated Hyperlipidemia in Streptozotocin-Induced Diabetic Rats

目的:研究佩兰中药颗粒对2型糖尿病大鼠糖脂代谢的改善作用,探讨中药佩兰对大鼠肝脏DGAT2表达的影响。方法:高脂饮食加尾静脉小剂量注射链脲佐菌素(STZ 28 mg/kg)建立2型糖尿病大鼠模型,将造模成功的合并脂代谢紊乱的2型糖尿病大鼠随机分为阳性对照组、佩兰中药颗粒组、吡格列酮组每组9只,另设空白对照组10只。佩兰中药颗粒组予佩兰中药颗粒按3 g/(kg·d)灌胃给药,吡格列酮组0.3 mg/(kg·d)灌胃,阳性对照组及空白对照组予0.9%氯化钠注射液灌胃,持续给药5周后,检测血清甘油三酯(serum triglyceride,TG)、血清总胆固醇(serum total cholesterol,TC)、游离脂肪酸(free fatty acid,FFA)、空腹血糖(fasting blood glucose,FBG)、空腹胰岛素(fasting insulin,FINS)并计算HOMA-IR评估大鼠胰岛素抵抗水平。应用RT-PCR与Western blot方法检测肝脏DGAT2基因及蛋白表达水平。结果:经治疗后,佩兰中药颗粒组大鼠的血清TG、TC水平与阳性对照组相比得到了明显的改善,肝脏DGAT2的基因及蛋白表达水平与阳性对照组相比下调。结论:佩兰可以改善由链脲佐菌素诱导的2型糖病大鼠脂代谢紊乱,其机制与中药佩兰下调大鼠肝脏中DGAT2基因与蛋白表达有关。

Objective： To investigate the effect and mechanism of the Eupatorium fortunei Turcz. extract onhyperlipidemia in streptozotocin-induced diabetic rats. Methods： High-fat diet combined with a low dose of streptozocin（ STZ 28 mg / kg） treatment was used for the establishment of T2DM-rats model. The T2 DM rats were randomly divided into three groups,includingmodel group（ Model）,extract treated group [3. 0 g /（ kg · d）,i. g],and Pio group [0. 3 mg /（ kg · d）,i. g]. Extract treated group and Pio groups were treated with different doses of Eupatorium fortunei Turcz. extract or Pioglitazone for 5 weeks,respectively. Meantime,normal saline was given to the control and model groups. All the rats were sacrificed and followed by the detection of fasting blood glucose（ FBG）,fasting serum insulin（ FINS）,triglyceride（ TG）,total cholesterol（ TC） and free fatty acid（ FFA）. Besides,the mRNA and protein expression level of DGAT2（ acyl CoA： diacylgycerol acyltransferase 2） in all groups were performed as well. Results： Our data demonstrated that the Eupatorium fortunei Turcz. extract dramatically ameliorated the T2 DM rats＇ hyperlipidemia induced by high-fat diet combined with STZ treatment on the TG and TC levels in plasma. Meanwhile,the DGAT2 mRNA expression was down regulated as well as the protein expression. Conclusion： Collectively,extract from the Eupatorium fortunei Turcz. distinctly improved the T2 DM rats induced by high-fat diet combined with STZ treatment by down regulating the expression of DGAT2.