Wilfoside C3N Promotes Tumor Cell Death by Activating Gammadelta T Cells-Mediated Anti-tumor Immunity

Vδ1^+γδ T lymphocytes are known to play important roles in anti-tumor immunity.We recently reported an anti-tumor activity of wilfoside C3 N,an active component extracted from Chinese medicinal herbs.In the current study,we evaluated the role of Vδ1^+γδ T cells in C3 N anti-tumor activity using an in vitro cell co-culture model.We found that C3 N induced the ECA109 tumor cells to undergo apoptosis in the presence of Vδ1^+γδ T cells.The level of ECA109 apoptosis maximized when both C3 N and Vδ1 + γδ T cells were present,which correlated with the increased expression of Fas on ECA109 and Fas ligand on Vδ1^+γδ T cells induced by C3 N.In addition,C3 N also enhanced secretion of cytokines,perforin and granzymes by Vδ1^+γδ T cells.These observations suggest that activation of Vδ1^+ γδ T cells may play a critical role in C3N-mediated anti-tumor activity.

Vδ1＋ 79 T lymphocytes are known to play important roles in anti-tumor immunity. We recently reported an anti-tumor activity of wilfoside C3N, an active component extracted from Chinese medicinal herbs. In the current study, we evaluated the role of Vδ1＋ 79 T cells in C3N anti-tumor activity using an in vitro cell co-culture model. We found that C3N induced the ECA109 tumor cells to undergo apoptosis in the presence of Vδ1＋ γδ3 T cells. The level of ECA109 apoptosis maximized when both C3N and Vδ1＋ γδ T cells were present, which correlated with the increased expression of Fas on ECA109 and Fas ligand on Vδ1＋ γδ T cells induced by C3N. In addition, C3N also enhanced secretion of cytokines, perforin and granzymes by Vδ1＋ γδ T cells. These observations suggest that activation of Vδ1＋ γδT cells may play a critical role in C3N-mediated anti-tumor activity.