摘要
目的了解2014年广州市某工厂发生的一起非O1/O139群霍乱弧菌食物中毒分离株的耐药特性及分子特征。方法对分离到的6株非O1/O139群分离株进行生化鉴定、血清学鉴定、药物敏感性实验、毒力相关基因检测和脉冲场凝胶电泳(PFGE)分子分型分析。结果 6株分离株经生化鉴定、血清学鉴定为非O1/O139群。药物敏感性分析显示,6株分离株均对氨苄西林、头孢克肟、头孢拉定、阿米卡星、妥布霉素、庆大霉素和多粘菌素B耐药,对诺氟沙星、环丙沙星、复方新诺明、呋喃唑酮、四环素、吡哌酸、红霉素和氯霉素敏感。毒力基因聚合酶链反应检测结果显示,所有菌株均携带毒力表达调控基因(toxR)和溶血素基因(hly A),未检出霍乱肠毒素基因(ctx A)、毒力协同调节菌毛基因(tcp A)和肠毒素基因(ST);5株病例分离株和1株冷柜内壁分离株经限制性内切酶NotⅠ消化后的PFGE图谱为同一型别。结论此次食物中毒的病原体为非O1/O139群霍乱弧菌,具有共同的遗传特征,菌株出现了多重耐药,提示应加强该类菌株的监测,防止大范围扩散或暴发流行。
Objective To investigate the drug resistance and molecular characteristics of Vibrio cholera non-O1 / O139 strains isolated from a food poisoning in a factory in Guangzhou,Guangdong province in 2014. Methods Biochemical identification,serological identification,drug susceptibility test,virulence-associated gene detection and PFGE molecular typing on six V.choleraenon-O1 / O139 strains isolated from a food poisoning in Guangzhou were conducted. Results The biochemical identification and serological identification results indicated that the six strains were all V. cholera non-O1 / O139 strains; the drug susceptibility analysis showed that the 6 strains were resistant to ampicillin,cefixime,cefradine,amikacin,tobramycin,gentamycin and polymyxin B, but sensitive to norfloxacin, ciprofloxacin, cotrimoxazole, furazolidone, tetracycline,pipemidic acid,erythromycin and chloramphenicol; The PCR detection of virulence gene showed that all the strains were positive for toxR and hly A genes,but negative for ctxA,tcpA and ST genes; and the PFGE molecular typing of the strains digested by NotⅠshowed that five strains isolated from the cases and one strain isolated from the inner wall of a refrigerator were in the same type. Conclusion The pathogen of this food poisoning was V. cholera non-O1 / O139,which shared common genetic characteristics; and the strains were multi drug resistant. It is suggested to guide the clinical medication based on drug susceptibility test results to reduce the drug resistant strains.
出处
《疾病监测》
CAS
2017年第2期149-152,共4页
Disease Surveillance
