趋化因子CXCL16与脑梗死发病相关性研究

Relationship of chemokine CXCL16 and cerebral infarction

目的探讨趋化因子CXCL16与脑梗死发病的相关性。方法选取2014年3月至2015年8月在解放军463医院住院的脑梗死患者450例,设为观察组;收集同期在我院进行健康体检的人员450例,设为对照组。利用酶联免疫吸附法(ELISA)检测两组患者血浆趋化因子CXCL16表达变化;利用免疫组化检测收集的颈外动脉粥样硬化斑块剥脱术后患者的颈外动脉组织标本CXCL16的表达变化;利用测序的方法分析检测CXCL16基因rs3744700位点基因型及等位基因的分布情况。结果观察组患者血浆中CXCL16表达水平明显高于对照组,免疫组化染色分析显示颈外动脉粥样硬化斑块剥脱组织中CXCL16的表达水平明显增高,两组间比较,差异均有统计学意义(P<0.05)。观察组与对照组CXCL16基因rs3744700多态位点TT基因型和T等位基因频率比较,差异有统计学意义(P<0.05)。结论 CXCL16基因rs3744700多态位点TT基因型和T等位基因可能是脑梗死患者发病的一个独立危险因素。

Objective To explore the relationship between chemokine CXCL16 and the pathogenesis of cerebral infarction.Methods Tocollected patients with cerebral infarction from the March 2014 to August 2015 in No. 463 Hospital of PLA as the observation group（ n = 450）. At the same time,the subjects were collected for the physical examination as the control group（ n = 450）.ELISA analysis method was used to detect the plasma chemokine CXCL16 level between the two groups. The immunohistochemistry staining was used to detect the CXCL16 expression in carotid artery specimens. The sequencing analysis method was used to detect the CXCL16 rs3744700 genotype and allele frequency between the two groups. Results The plasma CXCL16 level in the observation group was significantly increased than the control group. Immunohistochemistry staining detected that the CXCL16 expression was incresed in carotid artery specimens. There was significant statistical difference of the TT genotype distribution frequency and T alleles frequency between the two groups（ P 0. 05）. Conclusion CXCL16 rs3744700 TT genotype and T allele may be independent risk factors for the cerebral infarction patients.