高尔基糖蛋白73在早期原发性肝癌中的表达及意义

Expression and significance of Golgi glycoprotein-73 in early hepatocellular carcinoma

目的研究高尔基糖蛋白73(GP73)在早期肝癌、慢性乙型肝炎和健康者血液及肝组织中的表达情况,通过比较探讨GP73在早期肝癌中的表达及意义。方法采用酶联免疫反应双抗体夹心(ELISA)法检测30例成年健康者(正常组)、30例慢性乙型肝炎患者(肝炎组)及50例早期肝细胞癌(肝癌组)患者血清中GP73的质量浓度,免疫组织化学法检测20例正常肝脏、30例慢性乙型肝炎及30例早期HCC组织中GP73蛋白的表达情况。结果 (1)肝癌组患者血清中GP73蛋白的质量浓度为(143.01±38.09)μg/L,显著高于正常组的(66.01±25.45)μg/L及肝炎组的(89.78±26.98)μg/L,且GP73蛋白在正常组、肝炎组及肝癌组血清中的质量浓度依次升高(均P<0.01);(2)GP73蛋白在肝癌组标本中的蛋白表达分别高于肝炎组及正常组,阳性率分别为83.33%、56.67%和15.00%(χ~2=5.08、22.74),肝炎组又高于正常组(χ~2=8.68),差异均有统计学意义(P<0.05)。结论 GP73蛋白在早期HCC中呈明显高表达,早期HCC患者血清中GP73蛋白的质量浓度明显高于慢性乙型肝炎及健康者;同时GP73蛋白在慢性乙型肝炎患者血清中的质量浓度亦高于健康者,提示GP73表达的升高可能与慢性HBV感染有关,可能参与了HCC的早期发生、发展。

Objective To compare the expression of Golgi glycoprotein-73（GP73） in blood serum and tissues of early hepatocellular carcinoma（HCC）, chronic hepatitis B（HBV） and healthy adults, and discuss the significance of GP73 in early HCC. Methods Enzyme-linked immunosorbent assay（ELISA） method was used to detect the concentration of GP73 in serum of 30 healthy adults（normal group）, 30 cases with HBV（hepatitis group） and 50 cases with early HCC（cancer group）. Immunohistochemical method was used to detect the tissue expression of GP73 in 20 cases of normal liver, 30 cases with chronic HBV and 30 cases with early HCC. Results（1） The serum concentration of GP73 in patients with early HCC was（143.01±38.09） μg/L, significantly higher than normal group（66.01±25.45） μg/L and hepatitis group（89.78±26.98） μg/L. And the concentration of GP73 increased gradually in normal, hepatitis and cancer groups（all P〈0.01）.（2） The expression of GP73 in early HCC tissues was higher than that in hepatitis and normal groups, the positive rates were 83.33%, 56.67% and 15.00%, respectively（χ~2=5.08, 22.74）. And the positive rate of hepatitis group was higher than normal group, the difference was statistically significant（χ~2=8.68, P〈0.05）. Conclusions GP73 shows high expression in the early HCC, and GP73 in serum of HCC is significantly higher than that of chronic HBV patients and healthy adults. Meanwhile, the concentration of GP73 in serum of patients with chronic HBV is higher than that of healthy adults, suggesting that the increased expression of GP73 may be related with chronic HBV infection, and the early occurrence and development of HCC may be involved in GP73.