三七总皂苷预处理对急性期缺血再灌注肾脏的保护作用

Protective effects of total saponins of Panax notoginseng(PNS)pretreatment on acute renal ischemia-reperfusion injury

目的探索三七总皂苷预处理对急性期肾缺血再灌注损伤的作用。方法取75只SD雄性大鼠,随机分为3组,每组25只,分为:假手术组(C组)、缺血再灌注损伤组(RIRI组)、三七总皂苷组(PNS组)。实验干预:(1)C组:实验前尾静脉注射与PNS组等量生理盐水,1次/d,共3d,麻醉后右肾切除,左肾暴露30min后关腹;(2)RIRI组:切除右侧肾脏,左侧肾动脉夹闭30min后恢复血流;(3)PNS组:实验前尾静脉注射PNS(150mg/kg),其余同RIRI组。在缺血再灌注前1h及再灌注后1,6,12,24h等5个时间点分别取5只大鼠,取血清测肌酐、尿素氮,肾组织匀浆检测肾组织超氧化物歧化酶活性(SOD)、丙二醛(MDA)含量,光镜下观察肾组织的病理变化,并分别进行比较。结果与C组比较,RIRI组的肌酐、尿素氮、MDA水平在再灌注24h内随着时间的延长逐渐升高,SOD活性则明显降低。PNS组的肌酐、尿素氮、MDA水平在再灌注6h后各组与RIRI组比较降低,SOD活性明显增强。结论 PNS对肾缺血再灌注损伤具有保护作用,其保护作用机制与增强SOD活性、清除自由基等有关。

Objective To observe the protective effects of total saponins of Panax notoginseng （PNS） on acute renal ischemia-reperfusion injury.Methods 75 male SD rats were randomly averaged into sham operation group,RIRI group and PNS group.Sham operation group was injected normal salineviatail vein 1 day 1 times （3 days） before experiment,and the right kidney was removed under general anesthesia,then the left kidney was exposed for 30 minutes,finally the incison was closed.RIRI group,the rats was injected normal salineviatail vein 1 day 1 times（3 days）before experiment,and their right kidneys were removed under general anesthesia,then the left renal artery was clamped for 30 minutes,and then the incison was closed.The rats of PNS group was injected PNS （150 mg/kg） via tail vein 1 days 1 times（3 days）before experiment,and the rest steps was the same with RIRI group.1h before experiment and after 1,6,12,24 h reperfusion,Blood and kidney samples were collected to examine the superoxide dismutase （SOD） activity,and malon dialdehyde （MDA）,serum creatinine （SCr）,blood urea nitrogen （BUN）,and the renal tissues were stained with HE and observed under light microscope.Results Compared with the sham operation group,renal ischemia reperfusion group,the serum creatinine,urea nitrogen,MDA levels within 24 h of reperfusion with the extension of time gradually increased,SOD activity was obviously decreased.PNS group of creatinine,urea nitrogen,MDA level in reperfusion after 6 h decreased,compared with RIRI group,SOD activity significantly enhanced.Conclusion PNS could protect kidney from ischemia-reperfusion injury,which may be related to tbe elimination of free radicals,increase SOD activities.