6种黄酮协同抗氧化作用及构效关系研究

The synergistic antioxidant effect and structure-activity relationship of six flavonoids

以ABTS^+·清除能力为指标,采用Chou-Talalay中效原理设计试验,研究6种结构不同的小分子黄酮协同抗氧化作用及构效关系。结果表明:6种黄酮化合物对ABTS^+·清除能力依次为:5,6,7-三羟基黄酮>6-羟基黄酮>3,7-二羟基黄酮>5-羟基黄酮>3-羟基黄酮>7-羟基黄酮>抗坏血酸,其中5,6,7-三羟基黄酮、6-羟基黄酮和3,7-二羟基黄酮氧化作用效果显著;在不同浓度下,两种黄酮复配物对ABTS+·清除能力的联合作用指数CI值有较大差异;3,7-二羟基黄酮和其它5种黄酮都有明显的协同作用。充分说明6种黄酮化合物之间的协同抗氧化作用与化合物的羟基位置和数量有关,具有C_(3,7)-二羟基是协同抗氧化作用的主要结构特点。

In this study,the ABTS free radicals scavenging activity was as an indicator,the Chou-Talalay method was used to investigate the synergistic antioxidant effect and structure activity relationship of six kinds of small molecules with different structures.The results showed that the ABTS free radicals scavenging activity of the six kinds of flavonoids was in following order,i.e.,5,6,7-trihydroxy flavone〉6-hydroxyl flavone〉3,7-dihydroxy flavone〉5-hydroxy flavone 〉3-hydroxy flavone 〉7-hydroxyl flavone 〉ascorbic acid.Among them,5,6,7-trihydroxy flavone,6-hydroxy flavone and 3,7-dihydroxy flavone oxidation effect significantly,under different concentration and two flavonoid compounds on ABTS free radicals scavenging activity of the combined effect of CI values are quite different,dihydroxy flavone and other five kinds of flavonoids have obvious synergistic effect.It was also found that the synergistic antioxidant effects of the six flavonoid compounds were related to the position and quantity of the hydroxyl group,the main structural feature of the synergistic antioxidant effect of 3,7-dihydroxy.