高灵敏黄曲霉毒素B_1免疫层析定量检测法的建立

Development of Quantitative Sensitive Immunochromatographic Assays for Detection of Aflatoxin B_1

以胶体金为标记物,借助于胶体金读取仪,建立了高灵敏的定量检测黄曲霉毒素B_1的胶体金免疫层析方法。试纸条制备时,当标记溶液pH为6.0,检测线全抗原的使用浓度为1.5mg/mL,金标抗体浓度为4μg/mL、用量为1.2μL时,所建立方法的灵敏度为5.7ng/L。通过加速保存试验,结果显示该试纸条的保存期大于1年。该试纸条应用于实际谷物及酱油加标样本检测,证明该方法可满足食品快速检测的需要。

The aim of this work was to establish a highly sensitive quantitative colloidal gold immunochromatographic assay with a test strip reader for the detection of aflatoxin B1 using colloidal gold as label.When the labeling pH was 6.0,the concentration of antigen on T-line was 1.5mg/mL,the concentration of antibody labeled on the colloidal gold was 4μg/mL,and the dosage of gold-labeled antibody was 1.2μL,the sensitivity of the assay was 5.7ng/L.The results of accelerated aging experiment indicated that the strips shelf time was more than a year.It was proved that this method can meet the needs of the rapid detection of food when the strip was applied to detect cereals and soybean sauce.