黄蜀葵花黄酮抗氧化性及对DNA氧化损伤的保护作用

The antioxidant activities and protection on DNA oxidative damage of flavonoid from the flowers of Abelmoschusmanihot

采用石油醚、乙酸乙酯和正丁醇对黄蜀葵花乙醇提取物进行分级萃取,得到石油醚相、乙酸乙酯相、正丁醇相和水相4个不同极性成分。采用DPPH·清除、·OH清除和H_2O_2清除试验考察了其抗氧化活性,研究了正丁醇相对·OH和H_2O_2诱导DNA氧化损伤的保护作用,并利用HPLC—MS对正丁醇中黄酮类物质进行了鉴定。结果表明:黄蜀葵花黄酮主要富集于乙酸乙酯相和正丁醇相;乙酸乙酯相对DPPH·的清除能力最好,IC_(50)值为0.038 6 mg/mL,而正丁醇相对·OH和H_2O_2的清除能力较好,IC_(50)值分别为0.225 0,0.575 7mg/mL;正丁醇相对·OH和H_2O_2诱导的DNA氧化损伤都有保护作用;正丁醇相中含有11种黄酮类化合物。

The ethanol extracts of flowers fromAbelmoschus manihot was extracted by petroleum ether、ethyl acetate and n-butyl alcohol,and four different polar portions were obtained.The scavenging effect of different phases on DPPH·,·OH and H2O2 were studied to research the antioxidant activities of each phase.Moreover,the protection of n-butyl alcohol phase on DNA oxidative damage was also studied,and the flavonoids component was identified by HPLC-MS.The results showed that the ethyl acetate phase had the best scavenging effect on DPPH·,with IC50 value 0.038 6mg/mL.However,the n-butyl alcohol phase had scavenge ·OH and H2O2 best,and the IC50 values were 0.225 0and 0.575 7mg/mL,respectively.The n-butyl alcohol phase was proved to show protective effect in some degree on DNA oxidative damage.,and totally 11 kinds of flavonoids were found in the n-butyl alcohol phase.