利托那韦对卡波氏肉瘤相关疱疹病毒感染细胞LYN表达的影响

Effects of Ritonavir on Expression of LYN in Kaposi's Sarcoma-associated Herpesvirus-infected Cells

目的探讨蛋白酶抑制剂利托那韦对卡波氏肉瘤相关疱疹病毒(Kaposi’s sarcoma associated herpesvirus,KSHV)感染细胞LYN表达的影响。方法原代培养人脐静脉内皮细胞HUVECs,从BCBL-1中提取KSHV病毒感染HUVECs,PCR扩增KS330233证实感染成功。CCK8法测定半数抑制浓度,CCK8法检测细胞增殖能力,Western blot检测LYN表达及p-PI3K和p-AKT水平。结果 RTV作用于细胞的IC_(50)为25μmol/L。RTV与LYN抑制剂PP2能抑制KSHV感染的HUVECs的增殖和LYN、p-PI3K和p-AKT的表达(P<0.05)。结论 RTV可通过抑制LYN及其下游的PI3K/AKT信号通路抑制KSHV感染细胞HUVECs的增殖。

Objective To detect the effects of protein inhibitor ritonavir（RTV） on the expression of LYN in Kaposi＇s sarcoma associated herpesvirus（KSHV） infected cells.Methods Primary Human Umbilical Vein Endothelial Cells（HUVECs） were cultivated by enzyme infusion method.KSHV were extracted from BCBL-1 cells and infected HUVECs.PCR was used to amplify KS330233 fragment to verify the KSHV infection.We used CCK8 method to detect the IC_（50） of RTV on HUVECs and the proliferation ability of the cells.Western blot was used to detect LYN expression,p-PI3 K and p-AKT levels.Results RTV could inhibit the growth of KSHV-infected HUVECs in a concentration-dependent manner（P〈0.05）.The IC50 of RTV effect on cells was 25μmol/L.RTV could inhibit LYN,p-PI3 K and p-AKT expression.Conclusion RTV could inhibit the proliferation of KSHV-infected HUVECs through inhibiting LYN and PI3K/AKT signaling pathways.