氟非尼酮干预大鼠皮肤瘢痕形成的实验研究

Effect of fluorofenidone on inhibiting scar formation in rat cutaneous wounds

目的:探索氟非尼酮(AKF-PD)在大鼠皮肤瘢痕形成的抑制作用及可能的作用机制。方法:建立大鼠线性瘢痕模型,在切口区涂抹AKF-PD外用制剂,观察其对创伤修复的影响;以成纤维细胞(FB)数目和胶原纤维密度为指标,考察药物对瘢痕形成的影响;以皮肤损伤部位中转化生长因子β1(TGF-β1)表达水平变化,探讨AKF-PD对瘢痕抑制作用的可能机制。结果:与空白对照相比,AKF-PD实验组明显延缓伤口愈合(P<0.01)、同时实验组FB的增殖和胶原密度明显降低(P<0.01);用药d 7,实验组皮损部位中TGF-β1表达较空白对照组显著降低(P<0.01)。结论:AKF-PD可抑制皮肤瘢痕的形成,其机制可能与抑制瘢痕形成过程中TGF-β1的表达有关。

Objective： To investigate the role of fluorofenidone in inhibiting scar formation in rat cutaneous wounds and the possible mechanisms. Methods： The model of scar in rat cutaneous wounds was established and AKF-PD was administered on the wounds to investigated its effect on wound healing progress. The pathomorphological changes of fibroblast and collagen was employed as indicators of the content of scar formation. The level of transforming growth factor β1（ TGF-β1） in scar tissue was monitored to explore the possible mechanism of AKF-PD in inhibiting scar formation. Results： Compared to the blank control group,the wound healing progress of AKF-PD group was obviously postponed accompanied with significant reduction of fibroblast proliferation and collagen deposition.And the level of TGF-β1in scar tissue was markedly decreased in the AFK-PD group. Conclusion： AKF-PD can inhibit scar formation in rat cutaneous wounds,and the mechanism might be related to inhibition of TGF-β1expression during the scar formation progress.