心脑舒通胶囊对蒙古沙鼠全脑缺血再灌注损伤模型的神经保护作用

Neuroprotective effects of Xinnaoshutong capsule on the brain ischemia-reperfusion injury in Mongolian gerbils

目的研究心脑舒通胶囊对蒙古沙鼠脑缺血再灌注(I/R)引发的脑损伤的保护作用。方法按照体重将沙鼠随机分为4组:假手术组、模型组、大小2个剂量(心脑疏通56,14 mg·kg^(-1))实验组,除假手术组外,其余各组采用结扎沙鼠双侧颈总动脉30 min、再灌注6d复制脑缺血模型。每天灌胃给药1次,持续给药7 d。通过HE染色观察海马CA1区神经元细胞的形态变化;免疫组化染色观察海马CA1区胶质纤维酸性蛋白(GFAP)的表达。结果与假手术组存活率(100%)相比,模型组的存活率为66.7%,明显降低,差异有统计学意义(P<0.01);与假手术组神经细胞比例(100.0±16.72)%相比,模型组的神经细胞比例为(45.56±16.72)%,差异有统计学意义(P<0.01);模型组脑组织中海马CA1区神经细胞排列稀疏紊乱且GFAP表达明显增多。与模型组的存活率为66.7%相比,实验组的存活率为86.7%,差异有统计学意义(P<0.01)。与模型组的神经细胞数目为(45.56±16.72)%比较,高剂量实验组细胞的比例为(79.84±15.39)%,差异有统计学意义(P<0.01);同时,心脑疏通组能抑制GFAP表达。结论心脑舒通胶囊能够提高缺血再灌注沙鼠的成活率,改善海马CA1区的神经细胞状态,并能抑制GFAP的表达。

Objective To investigate the protective effect and the possible mechanism of Xinnaoshutong capsule on brain damage in Mongolian gerbil exposed to brain ischemia.Methods Mongolian gerbils were divided into 4 groups randomly：sham operation group,model group,experimental-H and experimental-L groups（Xinnaoshutong 56,14mg·kg^-1）.Gerbil ischemia reperfusion（I/R） model was established by bilateral common carotid artery occlusion for 30 min followed by reperfusion for 6 days.Daily intragastric administration for 1 times,last for 7 days.The structure of neurons in hippocampal CA1 region was evaluated by Hematoxylin-Eosin（HE） staining.The expression of glial fibrillary acidic protein（GFAP） in the hippocampus CA1 area was evaluated by immunohistochemical staining.Results Compared with the sham operation group of survival rate（100%）,the gerbil survival rate of the 66.7%in model group significantly decreased（P〈0.01）.Compared with the sham operation group on nerve cell proportion of（100.00 ± 16.72）%,nerve cell proportion was（45.56 ± 16.72）%in model group with significantly decreased（P〈0.01）.The hippocampus CA1 area neurons in the brain to arrange sparse disorder in model group.The expression of GFAP increased obviously in model group.Compared with the model group on the survival rate of cerebral ischemia gerbils of 66.7%,can make the survival rate of cerebral ischemia gerbils in Xinnaoshutong group 86.7%increased significantly（P〈0.01）.And Xinnaoshutong can improve the hippocampus CA1 area neurons in the brain tissue form.Compared with the model group on the proportion of nerve cells of（45.56 ± 16.72）%,the proportion of nerve cells in experimental-H group（79.84 ± 15.39）%significantly increased（P〈0.01）.Xinnaoshutong can inhibit the expression of GFAP.Conclusion Xinnaoshutong has positively protective effect on brain ischemia-reperfusion injury,it may be relevant with inhibiting astrocyte activation and anti-apoptosis,which may be one of the mechanism of treatment of stroke.