不同清洗剂下内镜器械的消毒效果分析

Analysis on disinfection effect of endoscopic instrument by using different cleaning agents

目的通过不同清洗剂对内镜的消毒效果比较,探讨对内镜消毒效果最佳的清洗液。方法收集2014年3月至2015年6月份该院使用过的内镜328件,随机将所选的内镜分为实验组与对照组,两组均有内镜164件。实验组使用生物膜专用清洗剂进行清洗,对照组使用多酶清洗剂进行清洗,清洗后两组内镜均给予相同的灭菌流程。采用三磷酸腺苷(ATP)生物荧光技术检测两组清洗后内镜活检孔中ATP水平,采用目测法、残留蛋白检测法及咽拭子法来分析与统计两组内镜消毒的合格率。结果清洗后两组内镜活检孔中ATP水平较清洗前均明显减少,且实验组减少得更明显,差异有统计学意义(P<0.05);目测法、残留蛋白检测法及咽拭子法的检测结果显示,实验组内镜清洗的合格率明显高于对照组,差异均有统计学意义(P<0.05)。结论生物膜专用清洗剂能有效去除内镜腔内的生物膜,其对内镜的清洗效果更明显,值得在临床工作中大力推广应用。

Objective To investigate the optimal cleaning agents of endoscopic disinfection by comparing the disinfection effects of different cleaning agents.Methods Three hundreds and twenty-eight used endoscopes in our hospital from March 2014 to June2015were collected and randomly divided into the experimental group and control group,164 case sin each group.The experimental group were cleaned by biofilm special cleaning agents,while the control group used the multiple enzyme cleaner.After cleaning,the same sterilization process was given to the endoscopes in the two groups.Then the ATP level in the endoscopic biopsy hole was detected by adopting the ATP bioluminescence technique.The qualification rate of endoscopic disinfection was performed the statistical analysis by using the visual inspection method,residual protein assay and pharyngeal swab method.Results The ATP level of the endoscopic biopsy hole after cleaning in the two groups was significantly reduced compared with before cleaning,moreover which in the experimental group was more obvious,the difference was statistically significant（P〈0.05）;the results detected by the visual inspection method,residual protein assay and pharyngeal swab test showed that the qualification rate of endoscopic cleaning in the experimental group was significantly higher than that in the control group with statistical difference（P〈0.05）.Conclusion The biofilm special cleaning agents can effectively remove the biofilm of endoscopic cavity,moreover its cleaning effect on endoscopic is more apparent,which is worth to be promoted and applied in clinical work.