小细胞性肺癌患者检测抗纺锤体抗体和抗着丝点抗体的临床意义

Clinical significance of anti-mitotic spindle apparatus antibody( MSA) and anticentromere antibody detected in patients with small cell lung cancer(SCLC)

目的:研究抗纺锤体(MSA)和抗着丝点(ACA)抗体检测对诊断小细胞性肺癌(SCLC)的临床价值,为SCLC的分子研究提供临床依据。方法:对93例SCLC患者和208例非小细胞性肺癌(NO-SCLC)患者及50例健康体检者,用酶联免疫法定量检测MSA抗体,间接免疫荧光法定性检测MSA、ACA和抗核抗体(ANA),并对其结果进行回顾性分析。结果:1IIF法检测SCLC组MSA和ACA阳性率为36.56%和30.11%,均高于其他肿瘤组(P<0.01)。2相关性分析,MSA与SCLC的RR值高达12.93、12.74,ACA和ANA与SCLC的RR值为4.31和3.48。3MSA检测SCLC的ROC曲线下面积AUC为0.778,诊断价值中等。结论:MSA和ACA对SCLC有很强正相关危险因素,可能参与SCLC的发生发展,对SCLC的早期检测、临床诊断及潜在的治疗方法有一定的应用价值。

Objective： To detect anti-mitotic spindle apparatus antibody（ MSA） and anti-centromere antibody（ ACA） and explore the clinical value for the diagnosis of small cell lung cancer（ SCLC）,providing clinical evidence for molecular studies of SCLC. Methods： 93 SCLC patients,208 non-small cell lung cancer（ non-SCLC） patients and 50 healthy controls were enrolled in this study. MSA antibodies were detected by enzyme linked immunosorbent assay（ ELISA）. MSA,ACA and anti nuclear antibodies（ ANA）were examined by indirect immuno-fluorescence（ IIF）. And the results were retrospectively analyzed. Results： 1 The positivity for MSA and ACA by IIF assay was respectively 36. 56% and 30. 11% in SCLC group,higher than other tumor groups（ P〈0. 01）. 2 In correlative analysis,the RR value between MSA and SCLC was as high as 12. 93,12. 74,and the RR value of ACA and ANA with SCLC was respectively 4. 31 and 3. 48. 3the area under ROC（ Receiver operating characteristic） curve（ AUC） of MSA detection for SCLC was 0. 778,with medium diagnostic value. Conclusion： MSA and ACA for SCLC are highly strong positive correlation risk factors,and may participate in the occurrence and development of SCLC,moreover,have some application value for early detection,clinical diagnosis and potential treatments of SCLC.