有序介孔碳纳米球的合成、表征及载药控释研究

Study on synthesis, characterization and drug loading controlled release of ordered mesoporous carbon nanospheres

采用软模板法合成出介孔酚醛树脂球,通过部分碳化法获得有序介孔纳米球,考察了水浴反应时溶液的p H及模板剂对产物形态的影响,当反应溶液p H=8.0及模板剂为F127时,合成产物为规则纳米球状,球体大小均一且介孔明显。通过扫描电镜(SEM)及透射电镜(TEM)观察到产物表面有明显有序介孔存在,红外分析证明了其表面存在诸多活性基团。介孔碳纳米球成功负载阿霉素,载药率为38.2%,96 h后的药物释放率为58.5%。通过介孔碳材料与人成纤维细胞共培养以考察材料的生物相容性,利用吖啶橙(AO)染色观察细胞状态,采用MTT(3-(4,5-二甲基-2-噻唑)-2,5-二苯基溴化四氮唑噻唑蓝)法进行细胞增殖定量检测,结果表明:与介孔碳球共培养的细胞形态正常,结构完整,伪足清晰可辩,生长状况良好;共培养7 d后细胞存活率保持在80%以上,证明介孔碳纳米球有良好的生物相容性。

The mesoporous phenolic resin spheres were synthesized by using the soft template method. The ordered mesoporous nanospheres were obtained by using the partial carbonization method. The influence of the pH value of the solution and the template during the water bath reaction on the morphology of the product was investigated. When the pH value of the reaction solution is 8.0 and F127 template is used, the synthesized products are regular nanospheres with uniform size and obvious mesopores. By means of the scanning electron microscopy （SEM） and transmission electron microscopy （TEM）, it is observed that the obviously ordered mesopores exist on the surface of product. The infrared analysis confirms that many active groups exist on its surface. The doxorubicin was successfully loaded on the mesoporous carbon nanospheres, the loading rate is 38.2%, the releasing rate of the drug after 96 h is 58.5%. The bio-compatibility of the materials was investigated by co-culture of the mesoporous carbon materials with the human fibroblast. The cell state was observed by staining with acridine orange （AO）. The cell proliferation was quantitatively detected by using MTT （methylthiazolyldiphenyl-tetrazoliumbromide） method. The results show that the cell co- cultured with the mesoporous carbon spheres has normal morphology and integrated structure, the pseudopodium is distinct and readable, and the growth status is good. The survival rate of cell after co-culturing for 7 d remains above 80%, which proves that the mesoporous carbon nanospheres possess good bio-compatibility.