恶性胸腔积液患者可溶性簇分化抗原26和二肽蛋白酶Ⅳ的检测及临床意义

Detection and clinical significance of s CD26 and DPP-Ⅳ in patients with malignant pleural effusion

目的探讨恶性胸腔积液患者可溶性簇分化抗原26(s CD26)和二肽蛋白酶Ⅳ(DPP-Ⅳ)的水平变化及临床意义。方法选取良、恶性胸腔积液患者各86例作为研究对象,采用酶联免疫吸附试验(ELISA)法检测sCD26浓度和DPP-Ⅳ活性。结果恶性胸腔积液组患者的sCD26、DPP-Ⅳ水平显著高于良性胸腔积液患者,差异均有统计学意义(P<0.01)。胸腔积液sCD26曲线下面积(AUC)为0.91,灵敏度为86.5%,特异度为86.6%;胸腔积液DPP-ⅣAUC为0.90,灵敏度为82.7%,特异度为85.0%。Spearman相关分析显示,胸腔积液sCD26与DPP-Ⅳ水平呈显著正相关(95%CI:0.82~0.91,r=0.72,P<0.01)。结论 sCD26和DPP-Ⅳ的检测对恶性胸腔积液鉴别诊断有一定的临床参考价值。

Objective To investigate the changes and clinical significance of soluble cluster differentiation antigen 26 （ sCD26 ） and dipeptide protease Ⅳ （ DPP - Ⅳ） in patients with malignant pleural effusion. Methods 86 cases of benign and malignant pleural effusion were collected as the research objects, and enzyme linked immunosorbent assay（ELISA） was used to detect the concentration of sCD26 and the activity of DPP - Ⅳ. Results The levels of sCD26 and DPP -Ⅳin patients with malignant pleural effusion were significantly higher than those in benign pleural effusion, and the difference was statistically significant （ P 〈 0.01 ）. The area under the curve（AUC） of pleural effusion sCD26 was 0.91, the sensitivity was 86.5 %, specificity was 86.6% ; AUC of pleural effusion DPP - Ⅳ was 0.90, the sensitivity was 82.7% , specificity was 85.0%. Spearman correlation analysis showed the levels of pleural effusion sCD26 and DPP - Ⅳ were significantly correlated（95% CI： 0.82 -0.91, r = 0.72, P 〈 0.01 ）. Conclusion The detection of sCD26 and DPP - Ⅳ has a certain clinical reference value to the differential diagnosis of malignant pleural effusion.