甲胎蛋白异质体3、高尔基体蛋白73血清水平在评价原发性肝细胞癌射频消融治疗应答中的应用

Evaluation on the therapeutic response of radiofrequency ablation for primary hepatocellular carcinoma with the serum levels of AFP heterogeneity 3 and Golgi protein 73

目的评价甲胎蛋白异质体3(AFP-L3)、高尔基体蛋白73(GP73)血清水平检测在原发性肝细胞癌(HCC)射频消融治疗应答中的应用效果。方法收集入院前未接受过治疗的原发性肝癌患者34例(A组);肝炎、肝硬化患者46例(B组);健康体检者30例(C组)。A组患者经治疗后复查患者34例(D组)。检测总AFP和AFP-L3的含量,计算AFP-L3百分比;血清GP73采用酶联免疫法测定。结果原发肝癌患者治疗前施行AFP-L3百分比与GP73联合检测,阳性率明显高于AFP-L3百分比或GP73单项检测阳性率,差异有统计学意义(P<0.05);治疗后复检,AFPL3百分比阳性率和GP73阳性率均下降,差异均有统计学意义(P<0.05)。HCC患者治疗后血清中AFP-L3百分比由35.4%降至14.02%,而GP73则由243.13 ng/ml降至161.94 ng/ml,差异均有统计学意义(P<0.05)。结论 AFP-L3百分比与GP73联合检测诊断HCC,阳性率明显高于GP73或AFP-L3百分比单项检测。AFP-L3百分比和GP73检测可作为射频消融治疗HCC效果的有效判定指标。

Objective To investigate the effect of serum levels of AFP heterogeneity 3 （ AFP - L3 ） and Golgi protein 73 （ GP73 ） in the therapeutic response of radiofrequency ablation for primary hepatocellular carcinoma （HCC） patients. Methods 34 pa- tients with primary hepatocellular carcinoma who had not been treated before admission were selected as group A; 46 patients with hepatitis and cirrhosis were selected as group B, and 30 healthy controls were selected as group C. The 34 patients in group A who accepted thercpy and recheck were enrolled in group D. Total contents of AFP and AFP - L3 were detected, the ratio of AFP - L3 was determined and serum GP73 was determined by using enzyme - linked quantitative immunoassay. Results The patients with primary liver cancer were conducted for the combined detection of AFP - L3 and GP73, with the positive rate sig- nificantly higher than that of AFP - L3 or that of GP73, and the differences were statistically significant（ P 〈 O. 05 ）. After re - examination, the positive rates of GP73 and AFP - L3 decreased, with the differences statistically significant （ P 〈 O. 05 ）. The serum AFP - L3 of primary hepatocellular carcinoma patients decreased from 35.4% to 14.02%, while GP73 decreased from 243.13 ng/ml to 161.94 ng/ml, with the difference statistically significant（ P 〈 0.05 ）. Conclusion The positive rate. by the combined detection of AFP - L3 and GP73 is significantly higher than that of GP73 or that of AFP - L3 alone in diagnosis of primary HCC. The detection of AFP - L3 and GP73 can be used as an effective index for the diagnosis and prognosis in the treat- ment of HCC using radiofrequency ablation.