臭氧氧化预处理对大鼠肝缺血再灌注损伤的保护作用

Ozone oxidative intervention protects against hepatic ischemia reperfusion injury in rats

目的 评价臭氧氧化预处理对大鼠肝脏缺血再灌注损伤的影响.方法 将18只SD大鼠（雄性,8周龄,250 ～ 300 g）按随机数字表法分为3组：假手术组（S组）、缺血再灌注组（I/R组）和臭氧预处理组（O3＋ I/R组）,每组6只.O3＋I/R组于术前5天连续腹腔注射臭氧和氧气混合气体（臭氧浓度为50 mg/L,1 mg·kg^-1 ·d^-1）.采用结扎肝动脉、门静脉和胆管分支45 min后恢复灌注的方法制备大鼠肝脏缺血再灌注损伤模型.再灌注3h后抽取腹主动脉血样测定血清ALT和AST水平;处死大鼠取肝组织,测定超氧化物歧化酶（SOD）活性和丙二醛（MDA）含量.结果 与S组比较,I/R组和O3＋I/R组血清ALT和AST水平、肝组织MDA含量升高,I/R组肝组织SOD活性降低（P＜0.05）,O3＋ I/R组肝组织SOD活性升高（P＜0.05）;与L/R组比较,O3＋ I/R组血清ALT和AST水平、肝组织MDA含量降低,肝组织SOD活性升高.结论 臭氧预处理可减轻大鼠肝脏缺血再灌注损伤,其机制可能与抑制脂质过氧化反应有关.

Objective To explore the protective effect of ozone oxidative preconditioning on hepatic ischemia reperfusion injury in rats.Methods Eighteen 8-week-old SPF Sprague-Dawley male rats weighting 250 ～300 g were randomly divided into three groups （n =6 each）：sham operation group （group S）,ischemia/reperfusion group （group I/R） and ozone oxidative preconditioning group （group O3 ＋ I/R）.In Group O3 ＋ I/R,rats received five-day preconditioning treatments by intraperitoneal injection of ozone and oxygen mixed gases （ozone concentration 50 mg/L,1 mg · kg^-1 · d^-1）,and then experienced the procedure of hepatic ischemia/reperfusion injury.Model of hepatic ischemia/reperfusion injury was established by clamping the branches of hepatic artery and portal vein in the median and left lateral hepatic lobes for 45 min,followed by 3-h reperfusion.After reperfusion,blood samples were taken from the aorta abdominalis for detecting serum aminotransferases （ALT ＆ AST）.These rats were executed and the hepatic tissue samples were collected for measuring hepatic malondialdehyde （MDA） and superoxide dismutase （SOD） level.Results Compared with group S,concentrations of serum ALT,AST and hepatic MDA were increased in group I/R and O3 ＋ I/R;concentrations of hepatic SOD were decreased （P 〈 0.05） in group I/R,but concentration of hepatic SOD was increased in group O3 ＋ I/R.Compared with group IR,concentrations of serum ALT,AST and hepatic MDA were decreased,while concentration of hepatic SOD was increased in group O3 ＋ I/R （P 〈 0.05）.Conclusion Ozone oxidative preconditioning could inhibit the lipid peroxidation to protect the rats against hepatic ischemia/reperfusion injury.