大豆苷元对骨质疏松模型大鼠拔牙后牙槽窝骨修复的影响

Effects of Daidzein on Bone Rebuiling of Alveolar Fossa after Tooth Extraction in Osteoporotic-model Rats

目的:研究大豆苷元(Daidzein,Da)对骨质疏松模型大鼠拔牙后牙槽窝处骨修复的影响。方法:24只3月龄大鼠随机均分为4组:假手术组(Sham)、雌激素组(EG)、大豆苷元组(Da)(75 mg/kg·d-1)和去卵巢组(Ovx)。摘除卵巢1周后,所有大鼠拔去双侧上颌第一磨牙,第12周检测血清钙、磷含量及总碱性磷酸酶(alkaline phosphatase,ALP)活性,制作左侧上颌骨拔牙窝处骨组织切片,于光镜下观察牙槽窝骨修复情况。结果:去卵巢后大鼠血清钙含量(2.10±0.04)及ALP活性(3.47±0.36)显著性降低(P<0.01),大豆苷元能显著提高去卵巢后大鼠血清钙含量(2.27±0.05)及ALP活性(4.49±0.38)(P<0.01)。骨形态观察表明大豆苷元对于牙槽窝的骨保存功能与雌激素相似,与Sham组骨形态结构较接近。结论:大豆苷元能有效改善骨质疏松模型大鼠拔牙后血清钙含量和ALP水平,减少牙槽窝骨吸收并恢复骨形态。

Objective：To explore the effect of Daidzein（Da）on the alveolar bone rebuilding after tooth extraction in osteoporotic-model rats.Methods：Twenty-four 3-months-old female SD rats were randomly divided into four groups：sham group（Sham）,estrogen group（EG）,ovariectomized group（Ovx）,ovariectomized and Daidzein group（Da）.Bilateral maxillary first molars of all rats were extracted at the 7th day post ovarian surgery.The values of serum calcium,phosphorus and alkaline phosphatase（ALP）were recorded at the 12 th week.Meanwhile,bone tissue sections of the left side of alveolar bone were made to observe bone rebuilding.Results：The value of serum calcium（2.49±0.02）and ALP（5.31±0.5）of ovariectomized rats decreased significantly（P〈0.01）,Daidzein could significantly improve the value of calcium（2.27±0.05）and the activity of ALP（4.49±0.38）in serum of ovariectomized rats（P〈0.01）.Morphological observation in alveolar bone tissue around the tooth fossa showed that the function of bone preservation of Daidzein was similar to estrogen,and was relatively similar to that of Sham group.Conclusion：Daidzein can remarkably improve the value of calcium and ALP in serum,reduce resorption of alveolar bone around the tooth fossa,and rebuild the bone morphology after extraction in osteoporotic-model rats.