ALDH1A1在舌癌及淋巴结中的表达及其临床意义

Expression and Clinical Significance of ALDH1A1 in Tongue Squamous Cell Carcinoma and Lymph Node

目的:检测乙醛脱氢酶1A1(aldehyde dehydrogenase1A1,ALDH1A1)在舌鳞状细胞癌(tongue squamous cell carcinoma,TSCC)原发灶及颈淋巴结标本中的表达水平,分析其与患者临床病理特征及预后的关系。方法:采用免疫组化SP法检测50例舌鳞状细胞癌原发灶和10例正常舌组织、29例转移淋巴结和9例未转移淋巴结标本中ALDH1A1的表达水平。结果:舌鳞状细胞癌原发灶中ALDH1A1表达高于正常舌组织,差异有统计学意义(P<0.05);转移淋巴结中ALDH1A1表达高于未转移淋巴结,差异有统计学意义(P<0.05)。在舌鳞状细胞癌原发灶中,ALDH1A1表达与患者的临床分期、有无淋巴结转移、原发灶大小及组织学分级有关(P<0.05);在淋巴结中,ALDH1A1表达与患者的临床分期、有无淋巴结转移及原发灶大小有关(P<0.05)。Kaplan-Meier分析表明舌鳞状细胞癌原发灶ALDH1A1高表达提示不良预后。结论:ALDH1A1的表达与舌鳞状细胞癌的生物学行为及预后密切相关,有望作为舌鳞状细胞癌预后相关因子,可能为舌鳞状细胞癌提供新的治疗靶点。

Objective：To detect the expression of aldehyde dehydrogenase1A1（ALDH1A1）in the primary lesions and lymph nodes of tongue squamous cell carcinoma（TSCC）patients,and analyze the their relationship with clinicopathological features and prognosis.Methods：The expression of ALDH1A1 was evaluated by SP immunohistochemistry in 50 cases of TSCC primary lesions,10 cases of normal tongue tissue and 29 metastatic lymph nodes,9non-metastatic lymph nodes.Results：Expression of ALDH1A1 in TSCC primary lesions was significantly higher than that in normal tongue tissue（P〈0.05）.Expression of ALDH1A1 in metastatic lymph nodes was significantly higher than that in non-metastatic lymph nodes（P〈0.05）.In primary TSCC lesions,the expression level of ALDH1A1 was associated with clinical stage,lymph node metastasis,tumor size and histological grade（P〈0.05）.In lymph nodes,the expression level of ALDH1A1 was associated with clinical stage,lymph node metastasis and tumor size（P〈0.05）.The Kaplan-Meier analysis demonstrated that ALDH1A1 overexpression was associated with poor prognosis in TSCC patients.Conclusion：ALDH1A1was closely correlated with the biological behavior and prognosis of patients in TSCC,and may provide new therapeutic targets for TSCC.