鸡骨髓源树突状细胞体外转化培养

Inducing and Differentiating Chicken Bone Marrow Dendritic Cells in Vitro

为深入探讨家鸡获得性免疫应答的分子机制,本研究探索了体外定向诱导和分化鸡骨源树突状细胞(Dendritic cell,DC)的方法。通过体外分离雏鸡骨髓源细胞,加入重组的鸡粒细胞-巨噬细胞集落刺激因子(GM-CSF)和白介素4(IL-4)定向诱导培养获得大量未成熟DCs,经过接种新城疫病毒(Newcastle disease virus,NDV)而激活,对其进行细胞形态学鉴定。结果表明:诱导4d后,部分细胞由单一的圆形球状逐渐生长呈短梭状;诱导6d后,细胞在40倍镜下可见基本呈短梭状且部分伸出触手;接种NDV后,细胞体积增大,触手伸长,形态趋于成熟,说明已成功建立体外利用细胞因子诱导分化鸡骨髓源DC的方法。

To further explore molecular mechanism of adaptive immune response in chicken, this study built a method of inducing and differentiating chicken bone marrow dendritic cells（DC） in vitro. Herein, the bone marrow cells were isolated from chicken in vitro, were induced a large amount of immature DCs with chicken GM-CSF and IL-4, and then were activated by Newcastle disease virus（NDV）. The results showed that chicken bone marrow cells were gradually grown from the round ball to the shape after 4 days of culturing, and the cells were short and some stretched out in 40 times of the lens after 6 days of culturing, and the cell volumes increased and the shape became mature after the NDV immunization. To sum up, the method of producing a large number of chicken DCs in vitro was successfully established.