荧光定量PCR在妊娠35～37周B族链球菌感染检测中的应用及妊娠结果分析

Rapid Detection of Group B Streptococcus by Real-time PCR in 35-37 Weeks of Gestation and the Maternal-neonatal Outcomes

目的运用荧光定量PCR法快速检测B族链球菌(GBS)感染并探讨其对妊娠结局及新生儿的影响。方法收集1235例妊娠晚期孕妇阴道拭子,采用细菌培养法和荧光定量PCR法检测是否感染GBS,并比较GBS阳性和阴性两组患者的妊娠结局。结果荧光定量PCR检测法阳性率显著高于细菌培养法(19.51%vs.9.72%),且差异有统计学意义。两组孕妇妊娠结局和两组新生儿出生后健康状况如宫内感染、新生儿感染等发生率比较差异有统计学意义。结论荧光定量PCR法检测GBS可早期预防治疗,有效改善孕妇妊娠结局和新生儿出生后健康状况,对孕晚期孕妇进行GBS感染的检测具有重要的临床意义。

Objective To evaluate if real-time PCR can be used the detection of group B streptococcus（GBS） in late pregnant women and to investigate the significance of GBS detection on the maternal-neonatal outcomes. Methods In this study, two vaginal-rectal swabs were collected and analyzed independently from 1235 pregnant women at 35-37 weeks of gestation by convenience sampling method. The specimens were subjected to GBS detection using real-time PCR assay or standard culture. The laboratory results were collected and the pregnant outcomes were followed. Results There was significant difference between real- time PCR and standard culture methods（ 19.51% vs. 9.72%, P 〈0.01 ）. Compared with the GBS negative group, the incidence of intrauterine infection, neonatal infection with pregnancy outcomes of pregnant women and the health status of newborns in the GBS positive group were significantly higher（ P 〈 0.05 ）. Conclusion Early detection of GBS with real-time PCR could promote the pregnancy outcomes of pregnant women and the health status of newborns. It is significant to carry out GBS screening among pregnant women in late pregnancy.