日本和牛Y染色体USP9Y基因多态性与父系起源研究

Paternal Origins and Polymorphism of Y Chromosome USP9Y Gene in Japanese Black Cattle

【目的】为了分析日本和牛Y染色体USP9Y基因遗传多态性与父系起源。【方法】利用PCR扩增与限制性酶酶切方法。【结果】对8头日本和牛纯种及19头和秦F1代杂种牛进行Y染色体USP9Y基因多态性及父系起源研究,结果表明:日本和牛与其杂种牛都具有USP9Y基因多态性,USP9Y基因的PCR产物均显示471bp和552bp两种带型,其中552bp带型不能被SspI酶酶切,则这2种带型对应Y1和Y2两种普通牛单倍型组,表明日本和牛含有Y1和Y2两个父系支系,有2个普通牛父系起源。日本和牛和杂种牛单倍型多样度分别为0.5714±0.0945和0.1988±0.1121,提示日本和牛具有较高的父系遗传多样性。【结论】日本和牛具有较高的父系遗传多样性及2个普通牛父系起源。

[Objective] To explore the genetic polymorphism of Y chromosome USP9Y gene and paternal origins in Japanese black cattle. [Method] Using PCR and restriction enzyme digestion method. [Result] The polymorphism of Y chromosome USP9Y gene and paternal origins of eight Japanese black cattle and 19 F1 hybrids from the hybridization between male Japanese black cattle and female Qinchuan cattle were analyzed. The results showed that Japanese black cattle and the hybrids indicated USP9Y gene polymor- phism with PCR products of 471 bp and 552 bp respectively, and the 552 bp PCR products can＇t be digested by SspI enzyme. Therefore, the above two band types represented Y1 and Y2 cattle haplogroups, respec- tively, indicating that Japanese black cattle had two patrilineal lineages with two taurine paternal origins. The haplotype diversities of Japanese black cattle and its hybrids were 0. 5714 ± 0. 0945 and 0. 1988 ±0. 1121 respectively, suggesting that Japanese black cattle has relatively high paternal genetic diversity. [Conclusion] Japanese black cattle had high paternal genetic diversity and two taurine paternal origins.