变应性鼻炎继发哮喘患者血浆microRNA表达谱的改变

Changes of plasma microRNAs expression profiles in allergic rhinitis patients with secondary asthma

目的应用microRNA表达谱芯片技术,选取临床明确诊断的变应性鼻炎(allergic rhinitis,AR)及变应性鼻炎罹患哮喘(asthma,AS)患者相应血浆,分析变应性鼻炎患者罹患哮喘后血浆microRNA的差异性表达。方法选取AR及AR+AS各3例,使用miRCURY LNATM芯片,筛选出两组患者血浆中差异表达的microRNA并进行聚类分析;最后通过生物信息学分析预测其靶基因。结果与AR组相比较,AR+AS组差异表达的microRNA共有9个,其中表达下调的有4个,表达上调的有5个。靶基因预测显示,以黏附连接、Wnt和MAPK信号传导通路靶基因的差异表达最为显著。结论通过microRNA芯片发现的相关差异性表达的microRNA,有可能成为AR患者罹患AS的易感性生物标志物及临床可能的治疗靶点,但是尚需要进一步靶基因验证及功能学分析。

Objective To detect the differential expressions of plasma microRNAs in allergic rhinitis （AR） patients with secondary asthma （AS） via microRNA expression profile chip technology. Methods Plasma samples were collected from patients suffering from AR （AR group）and AR patients with secondary AS （A18 ＋ AS group） respectively, and each group had 3 cases. The plasma microRNAs differentially expressed in AR ＋ AS group were screened out by nliRCURY LNATM chip and analyzed with cluster analysis. Finally, target genes were predicted by biological informatics technology. Results Compared with AR group, 9 microRNAs were differentially expressed in AR ＋ AS group. Of them, the expression was up-regulated in 4 and down-regulated in 5. Target gene prediction showed that target genes of adherens junction, Wnt and mitogen-activated protein kinase （MAPK） signaling pathways were differentially expressed most predominately. Conclusion The differentially expressed plasma microRNAs screened out by microRNA chip technology may be biomarkers of susceptibility in AR patients to AS and therapeutic targets for this disease.