玉屏风散对大鼠变应性鼻炎的作用研究

Study on the effect of Yupingfeng on allergic rhinitis in rats

目的观察玉屏风散在卵清蛋白致敏大鼠变应性鼻炎模型的抗过敏作用。方法实验在湖南省儿童医院动物实验室进行,30只6周龄雌性封闭群SD大鼠随机分成空白对照组(n=2)、对照组(n=4)、酮替芬灌服组(n=6)、玉屏风散灌服组(n=6)、玉屏分散滴鼻组(n=6)、布地奈德滴鼻组(n=6)5组。建立卵清蛋白诱导的大鼠变应性鼻炎大鼠模型,第1、8、15天,分别腹腔注射1 000μg卵清蛋白,自22 d开始300μg卵清蛋白滴每侧鼻孔。与此同时,造模第22天开始给药。采用叠加量化法记录各鼻部症状得分,然后计算总分。第37天,解剖获取胸腔心脏血、鼻中隔和双侧鼻腔外侧壁黏膜并且进行分析。结果实验各组与对照组比较,鼻部症状得分减少,纤毛上皮损伤较轻,血清中组胺水平显著降低,鼻黏膜中杯状细胞数量减少,嗜酸性粒细胞数量减少。结论玉屏风散对变应性鼻炎症状得分及鼻部组织具有抗过敏作用。

Objective To observe the antiallergenic effect of Yupingfeng （YPF） in rats with ovalbumin-sensitized allergic rhinitis （AR）. Methods Thirty female SD rats were randomly divided into five groups including blank control group （ n = 2）, control group （ n = 4）, Ketotifen group （ n = 6）, oral YPF group （ n = 6）, intranasal YPF group （ n = 6）, and intranasal budesonide group （ n = 6）. Ovabumin-induced animal model of AR was established in rats. Ovalbumin （ 1 000 ug/day） was administrated to the rats except those of the blank control group by intraperitoneal injection on days of 1, 8 and 15. Since the 22nd day, 300 ug of ovalbumin （diluted in15 ul of sterile normal saline） was applied intranasally per nostril. At the same time, medication treatment started and lasted for 2 weeks except in the blank control group. Superposition quantitative method was used to record the nasal symptom scores, and then total scores were calculated. At 37th day, all rats were executed. Abdominal aortic blood, mucous membranes were dissected from nasal septum and bilateral lateral nasal wall. All the specimens were analyzed. Results Compared with other groups, reductions in symptom scores, ciliated epithelium injury, serum histamine level, counts of eosinophil and goblet cell in nasal mucous membrane were observed in both oral and intranasal YPF groups. Conclusion Yupingfeng has anti-allergic effects on allergic rhinitis.