牙本质基质蛋白1在口腔鳞癌细胞中的表达

The Expression of Dentin Matrix Protein 1 in Oral Squamous Cancer Cells

目的:观察牙本质基质蛋白1(dentin matrix protein 1,DMP1)在舌鳞癌细胞Cal-27、Tca-8113以及人永生化表皮细胞Hacat中的表达情况,初步探讨DMP1与口腔鳞癌的相关性。方法:免疫荧光染色用于人永生化表皮细胞Hacat与舌鳞癌细胞Cal-27中,于共聚焦显微镜下观察DMP1在两组细胞的染色情况;采用Western blot方法,分别提取Hacat,Cal-27和人舌鳞癌细胞Tca-8113中DMP1蛋白并检测其含量。结果:通过免疫荧光实验观察到DMP1在Cal-27细胞及Hacat细胞中都表达于细胞质,同时发现DMP1在Hacat细胞中的荧光强度为41.2,高于Cal-27细胞荧光强度26.5;Western blot实验结果显示Hacat细胞中DMP1蛋白的灰度分析值为100和109,明显高于Cal-27和Tca-8113细胞的40.8和37.6。结论:DMP1在口腔鳞癌细胞和Hacat细胞中的表达存在差异,提示DMP1可能与口腔鳞癌的发生相关。

Objective： To detect the expression of dentin matrix protein 1 in tongue squamous cell carcinoma Cal-27, Tca-8113 and immortalized human epidermal cells Hacat, then analysis the relationship between DMP1 and oral squamous cell carcinoma. Methods：Immunofluorescence was used to detect the expression of DMP1 in Hacat and Cal-27 cells by the confocal microscopy. DMP1 protein would be extracted in squamous carcinoma Tca-8113, Cal-27 and Hacat cells respectively, the western blot was used to detect the protein content of DMP1 in the two groups. Results： In immunofluorescence, the expression of DMP1 protein in the cytoplasm, the fluorescence intensity of DMP1 in Hacat cells is 41.2, higher than the fluorescence intensity of DMP1 in Cal-27 cells is 26.5. Western blot experiments have shown, DMP1 protein fold change is 100 and 109 in Hacat cells, higher than DMP1 protein fold change is 40.8 and 37.6 in the Cal-27 and Tca-8113 cells. Conlusions： The expression between oral squamous cell carcinomas and normal tissue have differences,prompt DMP1 may be associated with the occurrence of oral squamous cell carcinomas.