生精片对精子DNA和顶体完整率及体外受精结局的影响分析

Analysis of influence by Shengjing tablets on sperm DNA,acrosome integrity rate and in vitro fertilization outcome

目的探讨精子顶体完整率、精子DNA完整率(DFI)、体外受精(IVF)结局与生精片之间的关系。方法 158例男性不育症患者作为研究对象,其中95例患者服用生精片治疗作为观察组,而其余63例服用左卡尼汀作为对照组,对比分析两组治疗情况,探讨生精片的临床应用价值。结果研究前,两组DFI、精子顶体完整率、前向运动精子(PR)数对比差异无统计学意义(P>0.05);研究3个月后,两组DFI显著低于研究前(P<0.05),精子顶体完整率、PR数显著高于研究前(P<0.05),观察组各项指标改善情况优于对照组,差异具有统计学意义(P>0.05)。观察组优胚率为(40.03±5.85)%,卵裂率为(91.15±11.57)%,受精率为(78.13±17.01)%,妊娠率为50.53%,对照组优胚率为(35.13±10.01)%,卵裂率为(91.50±12.08)%,受精率为(71.36±20.10)%,妊娠率为33.33%,观察组优胚率、受精率、妊娠率均优于对照组,差异具有统计学意义(P<0.05),两组卵裂率对比差异无统计学意义(P>0.05)。结论临床上针对不育症患者应用生精片治疗,可有效将患者DFI、精子完整率改善,对IVF结局具有促进作用,提高临床优胚率及妊娠率。

Objective To investigate relationship between sperm acrosome integrity rate, sperm DNA fragmentation index（DFI）, in vitro fertilization（IVF） outcome and Shengjing tablets. Methods Among 158 male patients as study subjects with infertility, there were 95 cases as observation group receiving Shengjing tablets and another 63 cases as control group receiving levocarnitine. Comparative analysis was made on treatment condition in both groups. Clinical application value of Shengjing tablets was investigated. Results Before research, there was no statistically significant difference of DFI, sperm acrosome integrity rate and PR count between the two groups（P0.05）. After 3 months of research, both groups had lower DFI than those before research（P0.05）, and their sperm acrosome integrity rate and progressive motility（PR） count were all higher than those before research（P0.05）. The observation group had better improvement of indexes than the control group, and their difference had statistical significance（P0.05）. The observation group had high qualified embryo rate as（40.03±5.85）%,cleavage rate as（91.15±11.57）%, fertilization rate as（78.13±17.01）%, and pregnancy rate as 50.53%. The control group had high qualified embryo rate as（35.13±10.01）%, cleavage rate as（91.50±12.08）%, fertilization rate as（71.36±20.10）%, and pregnancy rate as 33.33%. The observation group had all better high qualified embryo rate, fertilization rate and pregnancy rate than the control group, and their difference had statistical significance（P0.05）. There was no statistically significant difference of cleavage rate between the two groups（P0.05）. Conclusion Implement of Shengjing tablets in clinical treatment of infertility patients can effectively improve DFI and sperm integrity rate. This method shows acceleration effect for IVF outcome, and it can improve clinical high qualified embryo rate and pregnancy rate.