热带假丝酵母菌溶煤培养基的响应面优化

Medium response surfacey optimization of coal biosolubilization by Candida tropicalis

以热带假丝酵母菌对神府煤进行微生物溶煤实验,依次采用析因实验、最陡爬坡实验和响应面分析实验对溶煤培养基进行优化,并对溶煤产物的紫外可见光谱和红外光谱进行了分析。结果表明:有机氮源更利于溶煤作用,且最佳氮源为酵母粉;葡萄糖、酵母粉浓度和p H对溶煤作用影响显著,其最佳水平分别为8.152,15.759 g/L和7.414,此时的响应值A450达最大值14.937,溶煤率为23.03%;氧化煤被转化为深色小分子水溶性物质,与氧化煤的结构相似,溶煤产物中含有醛、酮、羧酸类化合物。

Biosolubilization tests of Shenfu coal were carried out with Candida tropicalis,single factor experiment,steepest ascent experiment and response surface analysis were used to optimize the medium for coal biosolubilization,and then,chemical properties of biosolubilization products were analyzed. Results show that glucose,yeast extract and p H were the most improtant factors that influence the process of biosolubilization,the optimum level of those three variate were 8. 152,15. 759 g/L and 7. 414,respectively,and the maximum response value（ A450） reached 14. 937 with an coal biosolubility of 23. 03%,oxidized coals were transferred into small molecules of water-soluble substances,and the structure of biosolubilization products is similar to oxidized coal,which have aldehydes,ketones and carboxylic acid compounds.