miR-375逆转MCF-7/Adr耐药性的功能研究

Effect of miR-375 on the resistance of MCF-7/Adr cells

目的研究乳腺癌耐阿霉素细胞株MCF-7/Adr高表达miR-375后对阿霉素敏感性的影响。方法将miR-375模拟物(miR-375 mimics)和mimics negative control(NC)分别转染MCF-7/Adr;阿霉素处理后用WST-1法检测耐阿霉素细胞的增殖情况;流式细胞仪检测细胞周期和凋亡;细胞划痕实验、transwell迁移、侵袭实验检测MCF-7/Adr细胞的迁移和侵袭能力。结果在MCF-7/Adr细胞中高表达miR-375可以提高其对阿霉素的敏感性并促进其凋亡;高表达miR-375的MCF-7/Adr细胞周期阻滞在G1期;转染miR-375后对MCF-7/Adr细胞的迁移、侵袭能力有抑制作用。结论 MCF-7/Adr细胞中高表达miR-375可以增加其对阿霉素的敏感性。

Objective To study the drug resistance of breast carcinoma MCF-7/Adr cells after transfection of miR-375. Methods The miR-375 mimics （ miR-375 mimics ） and mimic negative control （ NC ） were transfected into MCF-7/Adr cells. The proliferation of MCF-7/Adr cells was detected by WST-1 after adriamycin treatment. Cell cycle and apoptosis were detected by flow cytometry. Cell scratch test, transwell migration and invasion assay were used to detect MCF-7/Adr cell migration and invasion. Results Transfection of miR-375 increased the sensitivity of MCF-7/Adr cells to adriamycin and promoted cell apoptosis. Moreover, high level of miR-375 induced cell cycle arrest in G1 phase. Cell migration and invasion ability were also inhibited in MCF-7/Adr cells after miR-375 transfection. Conclusion High expression of miR-375 in MCF-7/Adr cells can enhance its sensitivity to adriamycin.