摘要
利用高效液相色谱法建立牛黄解毒制剂中胆红素的含量测定方法,色谱柱为Agilent Zorbax SB-C18色谱柱,流动相为乙腈—1%冰醋酸(95∶5),检测波长为450 nm.结果显示,胆红素进样量在0.8321~208.032 ng范围内具有良好的线性关系,r=0.9995,牛黄解毒片平均回收率为105.6%,RSD为1.42%,牛黄解毒丸平均回收率为96.2%,RSD为1.83%.结果表明,方法准确、简便,可作为牛黄解毒片及牛黄解毒丸等制剂中胆红素含量的测定方法.
The paper is going to establish a method for the determination of bilirubin in bezoar detoxicatio preparation. Liquid chromatography is used. The chromatography colmnn is Cls column; mobile phase is acetonitrile-1% acetic acid(95 : 5) ; the detection wavelength is 450 nm. The results show that the amount of bilirubin in the sample has a high linearity among the range of 0.8321 - 208.032 ng, r = 0.9995; the average recovery rate of the bezoar detoxicatio tablets is 105.6%, RSD = 1.42% ;the average recovery rate of bezoar detoxicatio pills is 96.2%, RSD = 1.83%. The results demonstrate that this method is accurate and simple, and can be used as the method to determine the bilirubin content in bezoar detoxicatio tablets and pills.
作者
罗亚玲
周娟
齐景梁
LUO Yaling ZHOU Juan QI Jingliang(Sichuan Institute for Food and Drug Control, Chengdu 610097, China)
出处
《成都大学学报(自然科学版)》
2017年第1期18-20,共3页
Journal of Chengdu University(Natural Science Edition)
关键词
牛黄解毒制剂
胆红素
液相色谱法
bezoar detoxication preparation
bilirubin
liquid chromatography