miR-34c在大鼠局灶性脑缺血模型中的表达

The expression of miR-34c in rat model of focal cerebral ischemia

目的通过检测miR-34c及其靶基因在大鼠局灶性脑缺血模型中的表达变化,初步探讨miR-34c及其靶基因在大鼠局灶性脑缺血中的作用。方法首先利用线栓法制备大鼠局灶性脑缺血模型;然后利用qRT-PCR检测miR-34c在大鼠脑缺血不同时间点的表达变化;利用生物信息学方法预测miR-34c的可能靶基因;再利用RT-PCR和qRT-PCR方法检测靶基因在大鼠脑缺血不同时间点的表达变化。结果成功制备大鼠局灶性脑缺血模型;miR-34c在大鼠脑缺血模型中随着缺血时间的延长其表达呈下降趋势;靶基因Hspa1b、Cacna2d1、Sperping1、Cntn2在大鼠脑缺血模型中随着缺血时间的延长其表达呈明显上升趋势。结论 miR-34c通过靶基因在大鼠局灶性脑缺血损伤过程中起到重要的保护作用。

Objective To detect the expression of miR-34c in middle cerebral artery occlusion （MCAO） model of rats and to preliminarily explore the function of miR-34c and its target genes in MCAO. Methads MCAO model of rats were prepared using thread embolism method. The expression of miR-34c was detected in model of MCAO at different times using qRT-PCR. The targets of miR-34c were predicted using bioinforma- tics methods. RT-PCR and qRT-PCR were further used to evaluated the expression of tin-gets in model of MCAO at different times. Results MCAO model of rat was successfully prepared. The expression of miR-34c was gradually decreased in model of MCAO at different times. However, predicated targets were obviously up-regulated in model of MCAO at different times. Conclusion miR-34c and its targets are involved in the process of MCAO which play an important protective role.