采用改良的克氏双糖铁琼脂培养基从漱口液中分离幽门螺杆菌的实验探索

Modified Kligler Iron Agar medium culture in isolation Helicobacter pylori from human mouth washing

目的探索高效、简捷、方便、低廉的方法从人群漱口液中分离幽门螺杆菌。方法被检者用生理盐水漱口,回收漱口液,离心,弃上清液,取沉淀物置脑心浸液培养基增菌培养。取菌液到改良的克氏双糖铁琼脂培养基中分离培养,挑取变红的菌落进行纯培养。对纯培养物进行染色检查和生化鉴定。结果漱口液中分离培养获得的纯培养物为幽门螺杆菌。结论用改良的克氏双糖铁琼脂培养基能比较快速准确地分离出漱口液中的幽门螺杆菌。

Objective To explore an efficient,simple,convenient,inexpensive method for isolating Helicobacter pylori from human mouth washing. Methods The participants used normal saline to rinse mouth,then their washing solution was collected and centrifugated,and the supernatant was removed.Then the sedimentation was put into brain heart infusion broth for enrichment culture.The bacterial fluid was put into the modified Kligler Iron Agar medium culture for isolation and culture,and the red cell colonies were selected for pure culture.Staining examination and biochemical identification were performed for the pure cultures. Results The isolated pure cultures obtained from human mouth washing were Helicobacter pylori. Conclusion Helicobacter pylori can be quickly and accurately isolated from the human mouth washing by the modified Kligler Iron Agar medium.