摘要
目的探讨活血通腑方对术后腹腔粘连大鼠粘连部位Nrf2、HO-1及NQO1蛋白及mRNA的影响。方法通过腹腔粘连评分评价腹腔粘连水平,Western blot法检测Nrf2核蛋白、HO-1和NQO1蛋白表达,qPCR检测Nrf2、HO-1和NQO1mRNA表达。结果活血通腑方能够显著降低肉眼腹腔粘连评分(P<0.01)。活血通腑方组Nrf2核蛋白、HO-1和NQO1蛋白表达以及mRNA表达显著升高(P<0.01)。结论活血通腑方对术后腹腔粘连大鼠粘连部位Nrf2、HO-1及NQO1表达有显著影响,可能通过调节Nrf2及下游因子的表达进而提升抗氧化应激能力,发挥抗腹腔粘连效应。
OBJECTIVE To investigate the effect of Huoxue Tongfu Formula on the expression of Nrf2, HO-1, NQO1 and mRNA in rats with postoperative peritoneal adhesion. METHODS Peritoneal adhesion was evaluated by gross abdominal adhesion score. The expressions of Nrf2, HO-1 and NQO1 were detected by western blot method. The expressions of Nrf2,HO -1 and NQO1 mRNA were tested via qPCR. RESULTS Huoxue Tongfu Formula could significantly reduce adhesion scores （P〈0.01）, manifested by evidently increased expression of Nrf2, HO-1 and NQO1 protein and mRNA（P〈0.01）. CONCLUSION The expression of Nrf2, HO-1 and NQO1 in the adhesions of rats with peritoneal adhesion is significantly affected by Huoxue Tongfu Formula, which is able to resist peritoneal adhesion by regulating Nrf2 and its downstream factors and enhancing the ability of anti-oxidative stress.
出处
《南京中医药大学学报》
CAS
CSCD
北大核心
2017年第2期150-154,共5页
Journal of Nanjing University of Traditional Chinese Medicine
基金
国家自然科学基金(81373843
81673982)
江苏省中医药局科技项目(YB2015002)
江苏省优势学科二期项目(YSHL2016-009)
关键词
腹腔粘连
氧化应激
Nrf2-ARE信号通路
postoperative peritoneal adhesion
oxidative stress
Nrf2-ARE signaling pathway
